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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2002-8-14
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pubmed:abstractText |
Hybridization kinetics were found to be significantly different for specific and non-specific binding of labeled cRNA to surface-bound oligonucleotides on microarrays. We show direct evidence that in a complex sample specific binding takes longer to reach hybridization equilibrium than the non- specific binding. We find that this property can be used to estimate and to correct for the hybridization contributed by non-specific binding. Useful applications are illustrated including the selection of superior oligonucleotides, and the reduction of false positives in exon identification.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-10521349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-10676951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-10921971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-10929718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-11236999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-11237012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-11283592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-11433338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-2748600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-2877398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-3822815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-5637197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-6696976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-6698006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-7171557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-7542923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-7569999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-7902321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-8850541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-8913616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-9056180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-9634850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12177314-9915496
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e86
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12177314-Base Pair Mismatch,
pubmed-meshheading:12177314-Exons,
pubmed-meshheading:12177314-Humans,
pubmed-meshheading:12177314-Jurkat Cells,
pubmed-meshheading:12177314-K562 Cells,
pubmed-meshheading:12177314-Kinetics,
pubmed-meshheading:12177314-Models, Chemical,
pubmed-meshheading:12177314-Nucleic Acid Hybridization,
pubmed-meshheading:12177314-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:12177314-RNA,
pubmed-meshheading:12177314-RNA, Messenger,
pubmed-meshheading:12177314-Retinoblastoma,
pubmed-meshheading:12177314-Sensitivity and Specificity,
pubmed-meshheading:12177314-Substrate Specificity,
pubmed-meshheading:12177314-Thermodynamics
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pubmed:year |
2002
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pubmed:articleTitle |
Use of hybridization kinetics for differentiating specific from non-specific binding to oligonucleotide microarrays.
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pubmed:affiliation |
Rosetta Inpharmatics, 12040 115th Avenue NE, Kirkland, WA 98034, USA.
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pubmed:publicationType |
Journal Article
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