Linked Life Data

12118158

Source:http://linkedlifedata.com/resource/pubmed/id/12118158

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2002-7-15
pubmed:abstractText
The distribution of glucose transporter (GLUT-1) and of interendothelial junction-associated proteins--zonula occludens protein (ZO-1), occludin, and beta-catenin--was studied using quantitative immunogold procedure. Lowicryl K4M-embedded samples of the cerebral cortex of 1-, 7-, and 14-day-, and 6-week-old (young-adult) mice were used. Ultrathin sections were exposed to specific rabbit polyclonal antibodies followed by colloidal gold-labelled secondary antibodies. We found that the density of immunosignals for GLUT-1 in both luminal and abluminal plasma membranes of the endothelial cells, and those closely related to the interendothelial junctions was low in blood microvessels from newborn mice, dropped slightly at the 7th day, and increased through the 14th day to the level of mature blood-brain barrier (BBB) observed in 6-week-old mice. The expression of ZO-1 was high in newborn mice and increased at the 7th day to the level similar to that found in 14-day- and 6-week-old mice. The expression of occludin was less intense than that of ZO-1 and increased from birth, reaching at the 14th day the level typical for mature BBB found in young-adult animals. The immunosignals for occludin were sparsely distributed inside the junctional clefts. Such a distribution indicates that the tight junctional characteristics are limited to a few short segments of the entire interendothelial cleft. The density of immunosignals for beta-catenin was lowest, and it had the tendency to a gradual, although inconsiderable, drop in the time course of BBB maturation. These findings suggest that the relatively high concentration of GLUT-1 in the interendothelial junctions results from the participation of abluminal plasma membranes of adjacent endothelial cells in the formation of the junctional complexes. The interendothelial junctions of newborn mice are equipped already with the main components of the tight junctions, and the concentration of these components (ZO-1, occludin) reaches the level of the mature BBB at the 14th day of postnatal life.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0300-4864
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
705-16
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12118158-Animals, pubmed-meshheading:12118158-Animals, Newborn, pubmed-meshheading:12118158-Blood-Brain Barrier, pubmed-meshheading:12118158-Cell Membrane, pubmed-meshheading:12118158-Cerebral Arteries, pubmed-meshheading:12118158-Cerebral Cortex, pubmed-meshheading:12118158-Cytoplasm, pubmed-meshheading:12118158-Cytoskeletal Proteins, pubmed-meshheading:12118158-Endothelium, Vascular, pubmed-meshheading:12118158-Female, pubmed-meshheading:12118158-Glucose Transporter Type 1, pubmed-meshheading:12118158-Immunohistochemistry, pubmed-meshheading:12118158-Male, pubmed-meshheading:12118158-Membrane Proteins, pubmed-meshheading:12118158-Mice, pubmed-meshheading:12118158-Mice, Inbred BALB C, pubmed-meshheading:12118158-Microscopy, Electron, pubmed-meshheading:12118158-Monosaccharide Transport Proteins, pubmed-meshheading:12118158-Phosphoproteins, pubmed-meshheading:12118158-Tight Junctions, pubmed-meshheading:12118158-Trans-Activators, pubmed-meshheading:12118158-beta Catenin
pubmed:year
2001
pubmed:articleTitle
Immunogold study of interendothelial junction-associated and glucose transporter proteins during postnatal maturation of the mouse blood-brain barrier.
pubmed:affiliation
New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, New York 10314, USA. Dobrogowska@msn.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't