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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2002-6-27
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pubmed:abstractText |
Vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs) are important regulators of blood and lymphatic vessel growth and vascular permeability. The VEGF-C/VEGFR-3 signaling pathway is crucial for lymphangiogenesis, and heterozygous inactivating missense mutations of the VEGFR-3 gene are associated with hereditary lymphedema. However, VEGF-C can have potent effects on blood vessels because its receptor VEGFR-3 is expressed in certain blood vessels and because the fully processed form of VEGF-C also binds to the VEGFR-2 of blood vessels. To characterize the in vivo effects of VEGF-C on blood and lymphatic vessels, we have overexpressed VEGF-C via adenovirus- and adeno-associated virus-mediated transfection in the skin and respiratory tract of athymic nude mice. This resulted in dose-dependent enlargement and tortuosity of veins, which, along with the collecting lymphatic vessels were found to express VEGFR-2. Expression of angiopoietin 1 blocked the increased leakiness of the blood vessels induced by VEGF-C whereas vessel enlargement and lymphangiogenesis were not affected. However, angiogenic sprouting of new blood vessels was not observed in response to AdVEGF-C or AAV-VEGF-C. These results show that virally produced VEGF-C induces blood vessel changes, including vascular leak, but its angiogenic potency is much reduced compared with VEGF in normal skin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiopoietin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Angpt1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vascular Endothelial...,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor C,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1530-6860
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pubmed:author |
pubmed-author:AlitaloKariK,
pubmed-author:ArolaJohannaJ,
pubmed-author:BuelerHansruediH,
pubmed-author:EnholmBerndtB,
pubmed-author:HytönenMaijaM,
pubmed-author:JeltschMichaelM,
pubmed-author:KarkkainenMarika JMJ,
pubmed-author:KerjaschkiDontschoD,
pubmed-author:PajusolaKatriK,
pubmed-author:SaaristoAnneA,
pubmed-author:TurunenPaïviP,
pubmed-author:VeikkolaTanjaT,
pubmed-author:Ylä-HerttualaSeppoS
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pubmed:issnType |
Electronic
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1041-9
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pubmed:dateRevised |
2011-8-3
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pubmed:meshHeading |
pubmed-meshheading:12087065-Adenoviridae,
pubmed-meshheading:12087065-Angiopoietin-1,
pubmed-meshheading:12087065-Animals,
pubmed-meshheading:12087065-Blood Vessels,
pubmed-meshheading:12087065-Capillary Permeability,
pubmed-meshheading:12087065-Cell Line,
pubmed-meshheading:12087065-Dependovirus,
pubmed-meshheading:12087065-Endothelial Growth Factors,
pubmed-meshheading:12087065-Genetic Vectors,
pubmed-meshheading:12087065-Lymphatic System,
pubmed-meshheading:12087065-Lymphokines,
pubmed-meshheading:12087065-Membrane Glycoproteins,
pubmed-meshheading:12087065-Mice,
pubmed-meshheading:12087065-Mice, Nude,
pubmed-meshheading:12087065-Nasal Mucosa,
pubmed-meshheading:12087065-Neovascularization, Physiologic,
pubmed-meshheading:12087065-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:12087065-Receptors, Growth Factor,
pubmed-meshheading:12087065-Receptors, Vascular Endothelial Growth Factor,
pubmed-meshheading:12087065-Skin,
pubmed-meshheading:12087065-Transfection,
pubmed-meshheading:12087065-Vascular Endothelial Growth Factor A,
pubmed-meshheading:12087065-Vascular Endothelial Growth Factor C,
pubmed-meshheading:12087065-Vascular Endothelial Growth Factors
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pubmed:year |
2002
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pubmed:articleTitle |
Adenoviral VEGF-C overexpression induces blood vessel enlargement, tortuosity, and leakiness but no sprouting angiogenesis in the skin or mucous membranes.
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pubmed:affiliation |
Molecular/Cancer Biology Laboratory and Ludwig Institute for Cancer Research, Biomedicum, University of Helsinki and Helsinki University Central Hospital, 00014 Helsinki, Finland.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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