Linked Life Data

12083326

Source:http://linkedlifedata.com/resource/pubmed/id/12083326

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
2002-6-26
pubmed:abstractText
The genetic resistance to nucleoside inhibitors of the reverse transcriptase (RT) of human immunodeficiency virus I (HIV-1) isolates in the Czech Republic was examined by a line probe assay (LiPA) and nucleotide sequencing. The results of LiPA analysis of 294 blood specimens obtained from 156 patients revealed a high incidence of mutations in the RT gene related to resistance to various drugs (67.3%) in various combinations. Mutations in RT gene (M41L, K70R and T215Y/F) conferring the resistance to zidovudine (ZDV) were most frequent (62.6%), that (M184V) responsible for the resistance to lamivudine (3TC) was less frequent (33.7%), while those linked to the resistance to dideoxyinosine (ddl) and dideoxyinosine together with dideoxycytidine (ddl/ddC) were rather rare (6.5% and 5.1%, respectively). LiPA gave a high rate of uninterpretable results due to codon hybridization failure, especially in HIV-1 isolates of non-B subtype. Thirty-two specimens were analyzed also by direct sequencing of a part of RT gene. The results obtained by LiPA and the sequencing were highly concordant for codons successfully analyzed by both methods, but the sequencing provided information also about the codons that could not be analyzed by LiPA. A high prevalence of resistant strains in the Czech Republic and their heterogeneity justifies a regular HIV-1 resistance testing. LiPA turned out as a fast, powerful and most reliable tool for such a purpose. However, due to an increasing diversity of HIV-1 strains circulating in the Czech Republic, LiPA cannot replace the nucleotide sequence analysis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0001-723X
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-86
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12083326-Base Sequence, pubmed-meshheading:12083326-Codon, pubmed-meshheading:12083326-Czech Republic, pubmed-meshheading:12083326-Didanosine, pubmed-meshheading:12083326-Drug Resistance, Viral, pubmed-meshheading:12083326-HIV Infections, pubmed-meshheading:12083326-HIV Reverse Transcriptase, pubmed-meshheading:12083326-HIV-1, pubmed-meshheading:12083326-Humans, pubmed-meshheading:12083326-Lamivudine, pubmed-meshheading:12083326-Molecular Probe Techniques, pubmed-meshheading:12083326-Molecular Sequence Data, pubmed-meshheading:12083326-Mutation, pubmed-meshheading:12083326-Phylogeny, pubmed-meshheading:12083326-Prevalence, pubmed-meshheading:12083326-Reverse Transcriptase Inhibitors, pubmed-meshheading:12083326-Sequence Analysis, DNA, pubmed-meshheading:12083326-Zalcitabine, pubmed-meshheading:12083326-Zidovudine
pubmed:year
2001
pubmed:articleTitle
Human immunodeficiency virus 1 strains resistant to nucleoside inhibitors of reverse transcriptase in isolates from the Czech Republic as monitored by line probe assay and nucleotide sequencing.
pubmed:affiliation
National Reference Laboratory on AIDS, National Institute of Public Health, Prague, Czech Republic.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't