Source:http://linkedlifedata.com/resource/pubmed/id/12079515
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2002-6-24
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| pubmed:abstractText |
We established a human lung cancer cell line, MI-4 from the pleural effusion of a 69-year-old male with advanced large cell undifferentiated carcinoma of the lung complicated by leukocytosis. The culture supernatant of MI-4 contained high levels of granulocyte colony stimulating factor (G-CSF). The intracellular localization of the G-CSF was identified by immunocytochemistry. Reverse transcription-polymerase chain reaction (RT-PCR) revealed G-CSF mRNA expression in this cell line. The cell line was successfully transplanted into nude mice. The transplanted nude mice also showed leukocytosis with a high serum G-CSF level. Southern blot analysis did not show amplification or rearrangement of the G-CSF gene in MI-4 cells. Spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) analyses revealed that this cell line has an additional chromosome 17 attached to a segment of chromosome 10 besides two intact chromosomes 17, and that each of these three chromosomes 17 has a G-CSF gene on chromosome 17q. Inflammatory cytokines, tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta, significantly enhanced G-CSF expression at both the protein and mRNA levels in MI-4. However, these cytokines did not stimulate the growth of MI-4 cells, regardless of abundant G-CSF production. TNF-alpha rather suppressed it, in a dose-dependent manner. Exogenous recombinant human G-CSF and anti-G-CSF antibody did not promote or inhibit the growth of MI-4 cells at any concentration examined. In addition, RT-PCR analysis did not show G-CSF receptor mRNA expression. These results suggest that this cell line does not have an autocrine growth loop for G-CSF. This cell line should be very useful for understanding the biological activity of G-CSF in G-CSF-overproducing lung cancer.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0910-5050
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
93
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
667-76
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:12079515-Aged,
pubmed-meshheading:12079515-Animals,
pubmed-meshheading:12079515-Blotting, Southern,
pubmed-meshheading:12079515-Carcinoma,
pubmed-meshheading:12079515-Chromosomes, Human, Pair 10,
pubmed-meshheading:12079515-Chromosomes, Human, Pair 17,
pubmed-meshheading:12079515-Dose-Response Relationship, Drug,
pubmed-meshheading:12079515-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:12079515-Humans,
pubmed-meshheading:12079515-Immunohistochemistry,
pubmed-meshheading:12079515-In Situ Hybridization, Fluorescence,
pubmed-meshheading:12079515-Interleukin-1,
pubmed-meshheading:12079515-Karyotyping,
pubmed-meshheading:12079515-Leukocytosis,
pubmed-meshheading:12079515-Lung Neoplasms,
pubmed-meshheading:12079515-Male,
pubmed-meshheading:12079515-Mice,
pubmed-meshheading:12079515-Mice, Inbred BALB C,
pubmed-meshheading:12079515-Mice, Nude,
pubmed-meshheading:12079515-Neoplasm Transplantation,
pubmed-meshheading:12079515-RNA, Messenger,
pubmed-meshheading:12079515-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12079515-Tumor Cells, Cultured,
pubmed-meshheading:12079515-Tumor Necrosis Factor-alpha
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| pubmed:year |
2002
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| pubmed:articleTitle |
Establishment and characterization of a new lung cancer cell line (MI-4) producing high levels of granulocyte colony stimulating factor.
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| pubmed:affiliation |
Department of Internal Medicine, Kochi Medical School, Kohasu, Nankoku, Kochi 783-8505.
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| pubmed:publicationType |
Journal Article
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