| pubmed-article:12050012 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12050012 | lifeskim:mentions | umls-concept:C0205102 | lld:lifeskim |
| pubmed-article:12050012 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:12050012 | lifeskim:mentions | umls-concept:C0028956 | lld:lifeskim |
| pubmed-article:12050012 | lifeskim:mentions | umls-concept:C0733755 | lld:lifeskim |
| pubmed-article:12050012 | lifeskim:mentions | umls-concept:C0600436 | lld:lifeskim |
| pubmed-article:12050012 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:12050012 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:12050012 | lifeskim:mentions | umls-concept:C0205349 | lld:lifeskim |
| pubmed-article:12050012 | lifeskim:mentions | umls-concept:C0205088 | lld:lifeskim |
| pubmed-article:12050012 | pubmed:issue | 2-3 | lld:pubmed |
| pubmed-article:12050012 | pubmed:dateCreated | 2002-6-6 | lld:pubmed |
| pubmed-article:12050012 | pubmed:abstractText | The presence of various modifications within oligomers changes their thermodynamic stability. To get more systematic data, we measured effects of 5- and 6-substituted uridine on thermal stability of (AUCU(Mod.)AGAU)2 and (AUCUAGAU(Mod.))2. Collected results lead to the following conclusions: (i) 5-halogenated and 5-alkylated substituents of the uridine affect thermal stability of the RNA duplexes differently. Moreover, the 5-fluorouridine changes stability of the RNA duplexes opposite to remaining 5-halogenouridines; (ii) for oligomers containing 5-chloro, 5-bromo or 5-iodouridine stronger hydrogen bond formed between oxygen-4 of the 5-halogenated uracil and 6-amino group of the adenine is presumably responsible for stabilizing effect; (iii) placing of A-U(5R) base pairs closer to the end of the duplex enhance thermal stability relatively to oligomer with central position of this base pair; (iv) the effects of 5-substituents are additive, particularly for substituents which stabilize RNA duplexes; (v) 6-methyluridines (N1 and N3 isomers) as well as 3N-methyluridine present at internal position of A-U(Mod.) inhibit duplexes formation; (vi) 6-methyluridines (N1 and N3 isomers) as well as 3N-methyluridine placed as terminal base pairs stabilize the duplexes mostly via 3'-dangling end effect. | lld:pubmed |
| pubmed-article:12050012 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12050012 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12050012 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12050012 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12050012 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12050012 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12050012 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12050012 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12050012 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:12050012 | pubmed:issn | 0301-4622 | lld:pubmed |
| pubmed-article:12050012 | pubmed:author | pubmed-author:Bia?aEwaE | lld:pubmed |
| pubmed-article:12050012 | pubmed:author | pubmed-author:ZiomekKrzyszt... | lld:pubmed |
| pubmed-article:12050012 | pubmed:author | pubmed-author:KierzekElzbie... | lld:pubmed |
| pubmed-article:12050012 | pubmed:author | pubmed-author:KierzekRyszar... | lld:pubmed |
| pubmed-article:12050012 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12050012 | pubmed:day | 19 | lld:pubmed |
| pubmed-article:12050012 | pubmed:volume | 97 | lld:pubmed |
| pubmed-article:12050012 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12050012 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12050012 | pubmed:pagination | 233-41 | lld:pubmed |
| pubmed-article:12050012 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:12050012 | pubmed:meshHeading | pubmed-meshheading:12050012... | lld:pubmed |
| pubmed-article:12050012 | pubmed:meshHeading | pubmed-meshheading:12050012... | lld:pubmed |
| pubmed-article:12050012 | pubmed:meshHeading | pubmed-meshheading:12050012... | lld:pubmed |
| pubmed-article:12050012 | pubmed:meshHeading | pubmed-meshheading:12050012... | lld:pubmed |
| pubmed-article:12050012 | pubmed:meshHeading | pubmed-meshheading:12050012... | lld:pubmed |
| pubmed-article:12050012 | pubmed:meshHeading | pubmed-meshheading:12050012... | lld:pubmed |
| pubmed-article:12050012 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12050012 | pubmed:articleTitle | The thermal stability of RNA duplexes containing modified base pairs placed at internal and terminal positions of the oligoribonucleotides. | lld:pubmed |
| pubmed-article:12050012 | pubmed:affiliation | Institute of Bioorganic Chemistry, Polish Academy of Sciences, 60-704 Poznañ, Noskowskiego 12/14, Poland. | lld:pubmed |
| pubmed-article:12050012 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12050012 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12050012 | lld:pubmed |
