Linked Life Data

12020639

Source:http://linkedlifedata.com/resource/pubmed/id/12020639

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-5-21
pubmed:abstractText
7-Ketocholesterol (7KC) is a major oxysterol found in atherosclerotic plaque and is believed to be derived both endogenously and exogenously (from the diet). Previously, we have demonstrated that subsequent to hepatic lipoprotein uptake, 7KC delivered in a model chylomicron remnant lipid emulsion is metabolised more rapidly and excreted into the intestinal tract and faeces to a much greater extent than simultaneously administered cholesterol. Furthermore, we have shown that human 7KC metabolism is dependent upon sterol 27-hydroxylase (27OHase). In the present work, we utilised a mouse model possessing the null mutation in the sterol 27-hydroxylase gene, Cyp27, to further investigate the metabolism and potential arterial accumulation of 7KC versus cholesterol. Despite the homozygous null mutation in Cyp27 (Cyp27-/-), 7KC was observed to undergo greater metabolism and excretion in the Cyp27-/- animals compared with the wild-type control mice. Six hours post-injection, 7KC levels were greater in aortae from Cyp27-/- mice but by 24 h, there was no significant difference between the knockout and control mice. We conclude that in contrast to humans, mice do not have an absolute requirement for 27OHase in order to metabolise 7KC and must rely on alternative side-chain oxidising pathways.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
1581
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-26
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Paradoxical enhancement of hepatic metabolism of 7-ketocholesterol in sterol 27-hydroxylase-deficient mice.
pubmed:affiliation
Cell Biology Group, Heart Research Institute, 145 Missenden Road, Camperdown, 2050 Sydney, Australia. mlyons@jax.org
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't