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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0027882,
umls-concept:C0035696,
umls-concept:C0205314,
umls-concept:C0332307,
umls-concept:C0439640,
umls-concept:C0679622,
umls-concept:C0949374,
umls-concept:C1171362,
umls-concept:C1424676,
umls-concept:C1515670
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pubmed:issue |
4
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pubmed:dateCreated |
2002-5-2
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pubmed:abstractText |
On the basis of a hypothesis that proteins encoded by the mRNAs that are transported to and translated at the dendrites/synapses may play key roles in synaptic plasticity, this study reports on attempts to isolate mRNAs which are localizing at the dendrites/synapses from mouse cerebellar synaptosomal fractions. Among 100 pieces of dendritic mRNA candidates, 10 pieces of mRNAs were found to contain the cytoplasmic polyadenylation element (CPE)-like sequences which were contained in certain mRNAs translated in dendrites. We next examined the issue of whether the CPE-like sequence-containing mRNAs (CPERs) were localized in the synapses/dendrites by means of in situ hybridization. The findings indicate that CPER9 was actually localized at the apical dendrites of a portion of cerebral cortex layer V pyramidal cells, as well as at the proximal dendrites of some of the cerebellar Purkinje cells. CPER9 was found to encode a mouse homolog of MRG15, a nuclear protein which contains a chromodomain identified in several proteins that act as regulators of transcription. Immunohistochemistry with anti-MRG15 antibodies revealed that MRG15 was localized in dendrites as well as in the nuclei of Purkinje cells. These results suggest that MRG15 may serve as a link between synaptic activity and gene expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0168-0102
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
299-308
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11985882-Animals,
pubmed-meshheading:11985882-Antibody Specificity,
pubmed-meshheading:11985882-Base Sequence,
pubmed-meshheading:11985882-Brain,
pubmed-meshheading:11985882-Cell Compartmentation,
pubmed-meshheading:11985882-Cerebellar Cortex,
pubmed-meshheading:11985882-Cerebral Cortex,
pubmed-meshheading:11985882-Dendrites,
pubmed-meshheading:11985882-Gene Expression,
pubmed-meshheading:11985882-Immunohistochemistry,
pubmed-meshheading:11985882-Male,
pubmed-meshheading:11985882-Mice,
pubmed-meshheading:11985882-Mice, Inbred C57BL,
pubmed-meshheading:11985882-Nuclear Proteins,
pubmed-meshheading:11985882-Presynaptic Terminals,
pubmed-meshheading:11985882-Protein Structure, Tertiary,
pubmed-meshheading:11985882-Purkinje Cells,
pubmed-meshheading:11985882-Pyramidal Cells,
pubmed-meshheading:11985882-RNA, Messenger,
pubmed-meshheading:11985882-RNA-Binding Proteins,
pubmed-meshheading:11985882-Sequence Homology, Amino Acid,
pubmed-meshheading:11985882-Transcription Factors,
pubmed-meshheading:11985882-mRNA Cleavage and Polyadenylation Factors
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pubmed:year |
2002
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pubmed:articleTitle |
A chromodomain-containing nuclear protein, MRG15 is expressed as a novel type of dendritic mRNA in neurons.
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pubmed:affiliation |
Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, 565-0871, Osaka, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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