Linked Life Data

11878714

Source:http://linkedlifedata.com/resource/pubmed/id/11878714

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2002-3-6
pubmed:abstractText
The present investigation evaluated the relationship between dysplasia of the uterine cervix and telomerase activity, expression of p53, MIB-1 and PCNA. Telomerase activity was measured on cervical cytobrush material from 126 women suspected of having dysplasia and 61 controls using the telomeric repeat amplification protocol. Immunohistochemistry was used to detect the tumor suppressor protein p53 and cell proliferation, the latter by MIB-1 and PCNA expression. Infection with human papillomavirus 16 was detected by PCR amplification and Southern blot hybridization of DNA extracted from the same brush material. Positive telomerase activity was found in 5 of 43 (11.6%) normal samples, 12 of 57 (21.1%) samples with inflammation or koilocytosis, 7 of 17 (41.2%) CIN 1 (cervical intraepithelial neoplasia, grade 1), 8 of 20 (40.0%) CIN 2, and 25 of 42 (59.5%) CIN 3/ CIS. Telomerase activity was significantly related to the level of dysplasia (p<0.001) and proliferation measured by MIB-1 (p=0.019), but not to the level of PCNA (p=0.445), HPV 16 status (p=0.098) or staining for p53 (p=0.271). Dysplasia was also related to PCNA, MIB1, p53, and presence of HPV 16. A sequential increase in the examined parameters, paralleling the progression of abnormality, was observed. PCNA and telomerase showed an increase in CIN 1, MIB-1 and HPV16 in CIN 2, and finally p53 in CIN 3/CIS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0903-4641
pubmed:author
pubmed:issnType
Print
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
607-17
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11878714-Adolescent, pubmed-meshheading:11878714-Adult, pubmed-meshheading:11878714-Antigens, Nuclear, pubmed-meshheading:11878714-Cervical Intraepithelial Neoplasia, pubmed-meshheading:11878714-Female, pubmed-meshheading:11878714-Humans, pubmed-meshheading:11878714-Ki-67 Antigen, pubmed-meshheading:11878714-Middle Aged, pubmed-meshheading:11878714-Nuclear Proteins, pubmed-meshheading:11878714-Papillomaviridae, pubmed-meshheading:11878714-Proliferating Cell Nuclear Antigen, pubmed-meshheading:11878714-Reproducibility of Results, pubmed-meshheading:11878714-Telomerase, pubmed-meshheading:11878714-Tumor Markers, Biological, pubmed-meshheading:11878714-Tumor Suppressor Protein p53, pubmed-meshheading:11878714-Uterine Cervical Dysplasia, pubmed-meshheading:11878714-Uterine Cervical Neoplasms
pubmed:year
2001
pubmed:articleTitle
Telomerase activity, MIB-1, PCNA, HPV 16 and p53 as diagnostic markers for cervical intraepithelial neoplasia.
pubmed:affiliation
Department of Clinical Biochemistry, Aarhus University Hospital, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't