Source:http://linkedlifedata.com/resource/pubmed/id/11857448
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2002-2-21
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| pubmed:abstractText |
Osteogenic Protein-1 (OP-1, BMP-7), a member of the bone morphogenetic protein family, stimulates synthesis of biochemical markers characteristic of the osteoblastic and chondrocytic phenotypes and induces new bone formation. Interleukin-6 (IL-6), a cytokine produced by a wide variety of cells, appears to interact with other factors producing different biological effects. In the present study, we showed that OP-1 action in fetal rat calvaria (FRC) cells was enhanced by the combination of IL-6 and the soluble receptor IL-6sR. OP-1 alone induced alkaline phosphatase (AP) activity by 4- to 5-fold above the control. Exogenous IL-6 soluble receptor (IL-6sR) synergistically stimulated the OP-1-induced AP activity and mineralized bone nodule formation by an additional 3-fold. The stimulation was IL-6sR concentration-dependent. The combination of IL-6 and IL-6sR synergistically stimulated OP-1 action by an additional 6- to 7-fold. BMPR-II receptor mRNA expression in FRC cells treated with OP-1 and IL-6 plus IL-6sR was stimulated further, while BMPR-IA, -IB, and ActR-I expressions were not affected. The intracellular signaling molecules Smad2 and Smad5 mRNA expressions were not changed under these conditions. The expression of selected BMP family members (BMP-3, -4, and -6) was altered in FRC cells treated with OP-1 in combination with IL-6 and IL-6sR. The combination of IL-6 and IL-6sR reduced the OP-1-stimulated BMP-3 mRNA levels and enhanced the suppressive effect of OP-1 on BMP-4 and -6 mRNA expressions. In conclusion, the present results demonstrate that exogenous IL-6 and IL-6sR synergistically stimulate OP-1 action in primary cultures of rat osteoblastic cells. One possible mechanism of synergy involves differential regulation of the effects of OP-1 on the expression of the type II BMP receptor and several other BMPs.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Bmp7 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 7,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Madh2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Smad2 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Smad5 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Smad5 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0021-9541
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2002 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
190
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
322-31
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:11857448-Alkaline Phosphatase,
pubmed-meshheading:11857448-Animals,
pubmed-meshheading:11857448-Bone Morphogenetic Protein 7,
pubmed-meshheading:11857448-Bone Morphogenetic Proteins,
pubmed-meshheading:11857448-Cell Differentiation,
pubmed-meshheading:11857448-Cells, Cultured,
pubmed-meshheading:11857448-DNA,
pubmed-meshheading:11857448-DNA-Binding Proteins,
pubmed-meshheading:11857448-Drug Synergism,
pubmed-meshheading:11857448-Embryo, Mammalian,
pubmed-meshheading:11857448-Gene Expression,
pubmed-meshheading:11857448-Interleukin-6,
pubmed-meshheading:11857448-Osteoblasts,
pubmed-meshheading:11857448-Phosphoproteins,
pubmed-meshheading:11857448-Rats,
pubmed-meshheading:11857448-Rats, Sprague-Dawley,
pubmed-meshheading:11857448-Receptors, Interleukin-6,
pubmed-meshheading:11857448-Skull,
pubmed-meshheading:11857448-Smad2 Protein,
pubmed-meshheading:11857448-Smad5 Protein,
pubmed-meshheading:11857448-Solubility,
pubmed-meshheading:11857448-Trans-Activators,
pubmed-meshheading:11857448-Transforming Growth Factor beta
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| pubmed:year |
2002
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| pubmed:articleTitle |
Osteogenic protein-1 and interleukin-6 with its soluble receptor synergistically stimulate rat osteoblastic cell differentiation.
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| pubmed:affiliation |
Department of Biochemistry, The University of Texas Health Science Center, San Antonio, Texas 78229-3900, USA. carolyeh@biochem.uthscsa.edu
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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