| pubmed-article:11781168 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11781168 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11781168 | lifeskim:mentions | umls-concept:C0143146 | lld:lifeskim |
| pubmed-article:11781168 | lifeskim:mentions | umls-concept:C0085732 | lld:lifeskim |
| pubmed-article:11781168 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:11781168 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:11781168 | lifeskim:mentions | umls-concept:C1327413 | lld:lifeskim |
| pubmed-article:11781168 | lifeskim:mentions | umls-concept:C0240618 | lld:lifeskim |
| pubmed-article:11781168 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11781168 | pubmed:dateCreated | 2002-1-8 | lld:pubmed |
| pubmed-article:11781168 | pubmed:abstractText | Ozone exposure can cause inflammation and impaired lung function. Human surfactant protein A (SP-A) may play a role in inflammation by modulating cytokine production by macrophages. SP-A is encoded by two genes, SP-A1 and SP-A2, and several allelic variants have been characterized for each gene. These allelic variants differ among themselves in amino acids that may exhibit differential sensitivity to ozone-induced oxidation and this may produce functional differences. We studied the effects of SP-A variants before and after ozone exposure on the production of tumor necrosis factor (TNF)-alpha and interleukin (IL)-8. These are important proinflammatory cytokines and are expressed by the macrophage-like THP-1 cells. Eight variants were expressed in vitro, characterized by gel electrophoresis, and studied. These included six single-gene SP-A alleles and two SP-A variants derived from both genes. Variants were exposed to ozone at 1 ppm for 4 hr at 37 degrees C, and we compared their ability to stimulate cytokine (TNF-alpha and IL-8) production by THP-1 cells to air-exposed and unexposed SP-A variants. We found that a) SP-A2 variants (1A, 1A(0), 1A(1) stimulate significantly more TNF-alpha and IL-8 production than SP-A1 variants (6A, 6A(2), 6A(4); b) coexpressed SP-A variants (1A(0)/6A(2), 1A(1)/6A(4) have significantly higher activity than single gene products; c) after ozone exposure, all SP-A variants showed a decreased ability to stimulate TNF-alpha and IL-8 production, and the level of the decrease varied among SP-A variants (26-48%); and d) human SP-A from patients with alveolar proteinosis exhibited a minimal decrease (18% and 12%, respectively) in its ability to stimulate TNF-alpha and IL-8 after in vitro ozone exposure. We conclude that biochemical and functional differences exist among SP-A variants, that ozone exposure modulates the ability of SP-A variants to stimulate cytokines by THP-1 cells, and that SP-As from bronchoalveolar lavage (BAL) fluid of certain alveolar proteinosis patients may be oxidized in vivo. | lld:pubmed |
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| pubmed-article:11781168 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:11781168 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11781168 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11781168 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:11781168 | pubmed:issn | 0091-6765 | lld:pubmed |
| pubmed-article:11781168 | pubmed:author | pubmed-author:WangGuirongG | lld:pubmed |
| pubmed-article:11781168 | pubmed:author | pubmed-author:UmsteadTodd... | lld:pubmed |
| pubmed-article:11781168 | pubmed:author | pubmed-author:PhelpsDavid... | lld:pubmed |
| pubmed-article:11781168 | pubmed:author | pubmed-author:Al-MondhiryHa... | lld:pubmed |
| pubmed-article:11781168 | pubmed:author | pubmed-author:FlorosJoannaJ | lld:pubmed |
| pubmed-article:11781168 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11781168 | pubmed:volume | 110 | lld:pubmed |
| pubmed-article:11781168 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11781168 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11781168 | pubmed:pagination | 79-84 | lld:pubmed |
| pubmed-article:11781168 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:11781168 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11781168 | pubmed:articleTitle | The effect of ozone exposure on the ability of human surfactant protein a variants to stimulate cytokine production. | lld:pubmed |
| pubmed-article:11781168 | pubmed:affiliation | Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA. | lld:pubmed |
| pubmed-article:11781168 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11781168 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11781168 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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