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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-12-28
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pubmed:abstractText |
We used mouse embryonic stem (ES) cells with systematic gene knockouts for DNA methyltransferases to delineate the roles of DNA methyltransferase 1 (Dnmt1) and Dnmt3a and -3b in maintaining methylation patterns in the mouse genome. Dnmt1 alone was able to maintain methylation of most CpG-poor regions analyzed. In contrast, both Dnmt1 and Dnmt3a and/or Dnmt3b were required for methylation of a select class of sequences which included abundant murine LINE-1 promoters. We used a novel hemimethylation assay to show that even in wild-type cells these sequences contain high levels of hemimethylated DNA, suggestive of poor maintenance methylation. We showed that Dnmt3a and/or -3b could restore methylation of these sequences to pretreatment levels following transient exposure of cells to 5-aza-CdR, whereas Dnmt1 by itself could not. We conclude that ongoing de novo methylation by Dnmt3a and/or Dnmt3b compensates for inefficient maintenance methylation by Dnmt1 of these endogenous repetitive sequences. Our results reveal a previously unrecognized degree of cooperativity among mammalian DNA methyltransferases in ES cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-10325416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-10344733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-10490608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-10555141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-10729832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-1423634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-1542678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-1606615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-2236038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-2427346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-2981636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-5136448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-6156004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-6269052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-7553622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-8065347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-8247133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9044832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9171109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9237905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9260521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9302295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9425235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9454602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9461416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9581283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9584187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9662389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9799591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11756544-9988266
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
480-91
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pubmed:dateRevised |
2010-5-21
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pubmed:meshHeading |
pubmed-meshheading:11756544-Animals,
pubmed-meshheading:11756544-Azacitidine,
pubmed-meshheading:11756544-Base Sequence,
pubmed-meshheading:11756544-Cell Line,
pubmed-meshheading:11756544-CpG Islands,
pubmed-meshheading:11756544-DNA,
pubmed-meshheading:11756544-DNA (Cytosine-5-)-Methyltransferase,
pubmed-meshheading:11756544-DNA Methylation,
pubmed-meshheading:11756544-Enzyme Inhibitors,
pubmed-meshheading:11756544-Kinetics,
pubmed-meshheading:11756544-Long Interspersed Nucleotide Elements,
pubmed-meshheading:11756544-Mice,
pubmed-meshheading:11756544-Mice, Knockout,
pubmed-meshheading:11756544-Repetitive Sequences, Nucleic Acid
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pubmed:year |
2002
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pubmed:articleTitle |
Cooperativity between DNA methyltransferases in the maintenance methylation of repetitive elements.
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pubmed:affiliation |
USC/Norris Comprehensive Cancer Center, Department of Urology, Keck School of Medicine of the University of Southern California, Los Angeles, California 90089-9181, USA.
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pubmed:publicationType |
Journal Article
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