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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-12-28
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pubmed:abstractText |
Receptor editing is a means by which immature bone marrow B cells can become self-tolerant. Rearrangements of heavy (H) and/or light (L) chain genes are induced by encounter with autoantigens to change the specificity from self to nonself. We have developed site-directed transgenic mice (sd-tg) whose transgenes code for the H chain of antibodies that bind DNA. B cells that express the transgenic H chain associate mainly with four of the 93 functional Vkappa genes of the mouse. Numerous aspartate residues that might inhibit DNA binding by the V(H) domain distinguish these L chain Vkappa sequences, but engaging these Vkappa editors often requires multiple rearrangements. Among the edited B cells is a subset of multispecific cells that express multiple receptors. One consequence of multispecificity is partial autoreactivity; these multispecific B cells may contribute to autoimmunity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1074-7613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
947-57
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11754816-Amino Acid Sequence,
pubmed-meshheading:11754816-Amino Acid Substitution,
pubmed-meshheading:11754816-Animals,
pubmed-meshheading:11754816-Antibodies, Antinuclear,
pubmed-meshheading:11754816-Antibodies, Monoclonal,
pubmed-meshheading:11754816-Autoantigens,
pubmed-meshheading:11754816-Autoimmunity,
pubmed-meshheading:11754816-B-Lymphocyte Subsets,
pubmed-meshheading:11754816-Codon,
pubmed-meshheading:11754816-DNA,
pubmed-meshheading:11754816-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:11754816-Gene Rearrangement, B-Lymphocyte, Light Chain,
pubmed-meshheading:11754816-Genes, Immunoglobulin,
pubmed-meshheading:11754816-Hybridomas,
pubmed-meshheading:11754816-Immunoglobulin Variable Region,
pubmed-meshheading:11754816-Immunoglobulin kappa-Chains,
pubmed-meshheading:11754816-Isoelectric Point,
pubmed-meshheading:11754816-Mice,
pubmed-meshheading:11754816-Mice, Inbred BALB C,
pubmed-meshheading:11754816-Mice, Inbred C57BL,
pubmed-meshheading:11754816-Mice, Transgenic,
pubmed-meshheading:11754816-Models, Molecular,
pubmed-meshheading:11754816-Molecular Sequence Data,
pubmed-meshheading:11754816-Mutagenesis, Site-Directed,
pubmed-meshheading:11754816-Mutation, Missense,
pubmed-meshheading:11754816-Polymerase Chain Reaction,
pubmed-meshheading:11754816-Protein Conformation,
pubmed-meshheading:11754816-Self Tolerance,
pubmed-meshheading:11754816-Sequence Alignment,
pubmed-meshheading:11754816-Sequence Homology, Amino Acid,
pubmed-meshheading:11754816-Transgenes
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pubmed:year |
2001
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pubmed:articleTitle |
Editors and editing of anti-DNA receptors.
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pubmed:affiliation |
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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