Linked Life Data

11753647

Source:http://linkedlifedata.com/resource/pubmed/id/11753647

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
52
pubmed:dateCreated
2001-12-25
pubmed:databankReference
pubmed:abstractText
Human ovarian cancer cells and tissues were examined for the presence or absence of a 42-bp splicing variant of ERCC1 gene, and for a possible functional role of this 42-bp sequence. This specific sequence exists in exon I, the 5'-UTR of the gene. Loss of this 42-bp sequence was associated with increased ERCC1 mRNA expression, in an assessment of 121 ovarian cancer specimens (p2<10(-6)). In cells in tissue culture, the absence of the 42-bp segment was associated with a twofold increased ability to drive transcription in a Luciferase reporter system. Protein can be demonstrated in ovarian cancer cells based on EMSA analysis. Computer analysis shows that this 42-bp sequence contains several binding sites, including a core-binding domain for protein RFX1, transcriptional repressor. These preliminary results lay the groundwork in determination of potential roles for a negative regulatory element in NER repair pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7694-8
pubmed:dateRevised
2008-9-5
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
An ERCC1 splicing variant involving the 5'-UTR of the mRNA may have a transcriptional modulatory function.
pubmed:affiliation
West Virginia University, Mary Babb Randolph Cancer Center, Robert C. Byrd Health Sciences Center, 1801 Health Sciences South, P.O. Box 9300, Morgantown, WV 26506-9300, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.