Linked Life Data

11689802

Source:http://linkedlifedata.com/resource/pubmed/id/11689802

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-11-5
pubmed:abstractText
PURPOSE: Myocardial angiogenesis may improve regional perfusion and perhaps function after cardiac injury. We evaluated the effect of endothelial cell transplantation into a myocardial scar on angiogenesis and ventricular function, as an alternative to angiogenic gene or protein therapy. Methods and Results: A transmural myocardial scar was created in the left ventricular free wall of rat hearts by cryoinjury. Allogeneic aortic endothelial cells were injected into the scar 2 weeks after cryoinjury. A cluster of transplanted cells was identified at the site of injection 1 day and 1 week after transplantation, but not after 2 weeks. The size of this cluster of transplanted cells decreased as vascular density in the transplanted scar tissue increased with time. Six weeks after transplantation, vascular density was significantly greater in transplanted hearts than in control hearts. Regional blood flow, by microsphere analysis, was greater in the transplanted rats. Systolic and diastolic ventricular function was similar between groups. In a second series of experiments, syngeneic aortic endothelial cells labeled with bromodeoxyuridine were transplanted 2 weeks after cryoinjury. Vascular density in the transplanted scar was greater than in controls. Labeled transplanted endothelial cells were identified forming part of the newly developed blood vessels. No difference in vascular density was found between allogeneic and syngeneic cell transplantation. Vascular endothelial growth factor was not expressed at greater levels in the transplanted cells or the myocardial scar. CONCLUSION: Transplanted endothelial cells stimulated angiogenesis, were incorporated into the new vessels, and increased regional perfusion in myocardial scar tissue, but did not improve global function in this cryoinjury rat model.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-5223
pubmed:author
pubmed:issnType
Print
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
963-71
pubmed:dateRevised
2009-11-3
pubmed:meshHeading
pubmed-meshheading:11689802-Animals, pubmed-meshheading:11689802-Cell Transplantation, pubmed-meshheading:11689802-Cyclosporine, pubmed-meshheading:11689802-Endothelial Growth Factors, pubmed-meshheading:11689802-Endothelium, Vascular, pubmed-meshheading:11689802-Heart Injuries, pubmed-meshheading:11689802-Immunosuppressive Agents, pubmed-meshheading:11689802-Lymphokines, pubmed-meshheading:11689802-Male, pubmed-meshheading:11689802-Myocardial Revascularization, pubmed-meshheading:11689802-Myocardium, pubmed-meshheading:11689802-Neovascularization, Physiologic, pubmed-meshheading:11689802-Protein Isoforms, pubmed-meshheading:11689802-Rats, pubmed-meshheading:11689802-Rats, Sprague-Dawley, pubmed-meshheading:11689802-Vascular Endothelial Growth Factor A, pubmed-meshheading:11689802-Vascular Endothelial Growth Factors
pubmed:year
2001
pubmed:articleTitle
Angiogenesis by endothelial cell transplantation.
pubmed:affiliation
Toronto General Hospital, Toronto General Research Institute, University Health Network, Department of Surgery, University of Toronto, Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't