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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-10-10
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pubmed:abstractText |
Reconstitution of G-protein-coupled receptor activated cation channels into the lipid bilayer was attempted with plasma membrane vesicles prepared from guinea-pig ileal smooth muscle using the purification technique previously applied to the large conductance Ca2+-dependent and ATP-sensitive K+ channels (Toro et al., 1990). Under Na+-rich conditions, incorporation of plasma membrane vesicles into the bilayer produced GTPgammaS (100 microM)-activatable channel activities that are inhibited by GDPbetaS (1 mM), sensitive to Ca2+ and enhanced by depolarization. The reversal potential and unitary conductance (tens of picosiemens) of these channels varied in a manner dependent on Na+ concentration, but not affected by Cl-. These results strongly indicate that the reconstituted channels activated by GTPgammaS belong to a class of voltage-dependent, Ca2+-sensitive cation-selective channels that are activated through a G-protein, and correspond most likely to the muscarinic receptor-activated cation channels previously identified in the same preparation. These results also suggest potential usefulness of bilayer incorporation technique to investigate the receptor-operated cation channels in smooth muscle.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0916-8737
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
39-51
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11592283-Animals,
pubmed-meshheading:11592283-GTP-Binding Proteins,
pubmed-meshheading:11592283-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:11592283-Guanosine Diphosphate,
pubmed-meshheading:11592283-Guinea Pigs,
pubmed-meshheading:11592283-Ileum,
pubmed-meshheading:11592283-Large-Conductance Calcium-Activated Potassium Channels,
pubmed-meshheading:11592283-Lipid Bilayers,
pubmed-meshheading:11592283-Muscle, Smooth,
pubmed-meshheading:11592283-Potassium Channels,
pubmed-meshheading:11592283-Potassium Channels, Calcium-Activated,
pubmed-meshheading:11592283-Sodium,
pubmed-meshheading:11592283-Thionucleotides
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pubmed:year |
2001
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pubmed:articleTitle |
Reconstitution in lipid bilayer of smooth muscle cation channels activated through a GTP-binding protein.
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pubmed:affiliation |
Department of Pharmacology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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