|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
39
|
|
pubmed:dateCreated |
2001-9-25
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
We previously identified a novel p53-induced mouse gene, wig-1, that encodes a 290 amino acid zinc finger protein (Varmeh-Ziaie et al., 1997). Here we have identified and characterized the human homolog of mouse wig-1. The human wig-1 protein is 87% identical to the mouse protein and contains three zinc finger domains and a putative nuclear localization signal. Human wig-1 mRNA and protein is induced following activation of wild type p53 expression in our BL41-ts p53 Burkitt lymphoma cells. Wig-1 is also induced in MCF7 cells following treatment with the DNA-damaging agent mitomycin C. Northern blotting detected low levels of wig-1 mRNA in normal human tissues. Fluorescence in situ hybridization mapped wig-1 to human chromosome 3q26.3-27. FLAG-tagged human wig-1 localizes to the nucleus. Ectopic overexpression of human wig-1 inhibits tumor cell growth in a colony formation assay. These results suggest that human wig-1 has a role in the p53-dependent growth regulatory pathway.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0950-9232
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
6
|
|
pubmed:volume |
20
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5466-74
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11571644-Amino Acid Sequence,
pubmed-meshheading:11571644-Animals,
pubmed-meshheading:11571644-Carrier Proteins,
pubmed-meshheading:11571644-Cell Division,
pubmed-meshheading:11571644-Cell Nucleus,
pubmed-meshheading:11571644-Chromosomes, Human, Pair 3,
pubmed-meshheading:11571644-Cloning, Molecular,
pubmed-meshheading:11571644-DNA Damage,
pubmed-meshheading:11571644-DNA-Binding Proteins,
pubmed-meshheading:11571644-Humans,
pubmed-meshheading:11571644-Mice,
pubmed-meshheading:11571644-Molecular Sequence Data,
pubmed-meshheading:11571644-Neoplasms,
pubmed-meshheading:11571644-Nuclear Proteins,
pubmed-meshheading:11571644-RNA, Messenger,
pubmed-meshheading:11571644-Rats,
pubmed-meshheading:11571644-Sequence Homology, Amino Acid,
pubmed-meshheading:11571644-Tissue Distribution,
pubmed-meshheading:11571644-Tumor Cells, Cultured,
pubmed-meshheading:11571644-Tumor Stem Cell Assay,
pubmed-meshheading:11571644-Tumor Suppressor Protein p53,
pubmed-meshheading:11571644-Up-Regulation,
pubmed-meshheading:11571644-Zinc Fingers
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Human wig-1, a p53 target gene that encodes a growth inhibitory zinc finger protein.
|
|
pubmed:affiliation |
Department of Oncology-Pathology, Cancer Center Karolinska (CCK), Karolinska Institute, SE-171 76 Stockholm, Sweden.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
