Source:http://linkedlifedata.com/resource/pubmed/id/11567902
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2001-9-24
|
| pubmed:databankReference | |
| pubmed:abstractText |
By using RT-PCR and 5'- and 3'-RACE methods, we isolated cathepsin B cDNA from hemocytes of Bombyx mori. The predicted open reading frame encoded 337 amino acids with 54% identity with the human cathepsin B, and 55% identity with the 29-kDa proteinase of the blowfly. Three active sites characteristic for cathepsin B were conserved in the deduced amino acid sequences of BmCtB cDNA at positions Cys-111, His-280 and Asn-300. Northern analysis identified a 1.3-kb mRNA. Expression of BmCtB was observed in the hemocytes from the day of wandering and was strongest at day 1 pupa. With in situ hybridization, strong signals were observed in the granular cells and plasmatocytes of day 0 pupa, but not in those of day 5 of the fifth larval instar. The results indicate another function of cathepsin B and insect hemocyte during metamorphosis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
1096-4959
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
130
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
393-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11567902-Amino Acid Sequence,
pubmed-meshheading:11567902-Animals,
pubmed-meshheading:11567902-Bombyx,
pubmed-meshheading:11567902-Cathepsin B,
pubmed-meshheading:11567902-Cloning, Molecular,
pubmed-meshheading:11567902-DNA, Complementary,
pubmed-meshheading:11567902-Gene Expression Regulation, Developmental,
pubmed-meshheading:11567902-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11567902-Hemocytes,
pubmed-meshheading:11567902-In Situ Hybridization,
pubmed-meshheading:11567902-Insect Proteins,
pubmed-meshheading:11567902-Metamorphosis, Biological,
pubmed-meshheading:11567902-Molecular Sequence Data,
pubmed-meshheading:11567902-RNA, Messenger,
pubmed-meshheading:11567902-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11567902-Sequence Homology, Amino Acid
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Isolation and expression of cathepsin B cDNA in hemocytes during metamorphosis of Bombyx mori.
|
| pubmed:affiliation |
College of Animal Sciences, Zhejiang University, 310029, Hangzhou, PR China
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|