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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2001-9-24
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pubmed:abstractText |
BrkA is a Bvg-regulated Bordetella pertussis protein that mediates serum resistance and adherence. It shares sequence identity with another B. pertussis virulence factor called pertactin, and it is a member of the diverse group of proteins found in Gram-negative bacteria that are secreted by an autotransporter mechanism. Sera, either from individuals who have been vaccinated with acellular pertussis vaccines, or from individuals who have no re-collection of recent infection with B. pertussis fail to kill wild-type B. pertussis, but kill brkA mutant strains very well. We examined whether BrkA could be neutralised in serum fitting this profile. BrkA is synthesised as a 103kDa precursor that is processed into a surface-associated N-terminal 73kDa passenger domain, and an outer-membrane embedded C-terminal 30kDa transporter moiety. Polyclonal antibodies were raised to a recombinant, re-folded histidine-tagged fusion protein representing the 73kDa passenger region. These anti-BrkA antibodies were shown to boost the existing bactericidal capacity of human serum against B. pertussis by neutralising BrkA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/BrkA protein, Bordetella pertussis,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Vaccine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
235-41
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11567769-Adult,
pubmed-meshheading:11567769-Animals,
pubmed-meshheading:11567769-Antibodies, Bacterial,
pubmed-meshheading:11567769-Antibody Specificity,
pubmed-meshheading:11567769-Antibody-Dependent Cell Cytotoxicity,
pubmed-meshheading:11567769-Antigens, Bacterial,
pubmed-meshheading:11567769-Bacterial Outer Membrane Proteins,
pubmed-meshheading:11567769-Blood Bactericidal Activity,
pubmed-meshheading:11567769-Bordetella pertussis,
pubmed-meshheading:11567769-Humans,
pubmed-meshheading:11567769-Immune Sera,
pubmed-meshheading:11567769-Neutralization Tests,
pubmed-meshheading:11567769-Peptide Fragments,
pubmed-meshheading:11567769-Pertussis Vaccine,
pubmed-meshheading:11567769-Protein Precursors,
pubmed-meshheading:11567769-Protein Processing, Post-Translational,
pubmed-meshheading:11567769-Rabbits,
pubmed-meshheading:11567769-Recombinant Fusion Proteins,
pubmed-meshheading:11567769-Specific Pathogen-Free Organisms
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pubmed:year |
2001
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pubmed:articleTitle |
Antibodies to BrkA augment killing of Bordetella pertussis.
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pubmed:affiliation |
Department of Microbiology and Immunology, University of British Columbia, #300-6174 University Boulevard, BC, V6T 1Z3, Vancouver, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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