Source:http://linkedlifedata.com/resource/pubmed/id/11500051
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2001-8-13
|
| pubmed:databankReference | |
| pubmed:abstractText |
Differentially expressed genes between normal liver and hepatocellular carcinomas were investigated using differential display. Consequently, we identified a fragment cDNA upregulated in tumor tissues. We screened the liver library and cloned the full-length cDNA, named F-LANa. Increased expression of F-LANa was confirmed by Northern blot analysis in 10 of 14 (71%) cases of hepatocellular carcinomas. Human F-LANa gene maps to chromosome 17p at D17S1828-D17S786, spans at least 11.8 kb, and contains 7 exons. This gene encodes a 239 aa protein exhibiting 97.9% similarity to the mouse ortholog gene, identified later by in silico cloning. Homology analysis was carried out in various species and showed that F-LANa was evolutionarily conserved from yeast to human. In addition, F-LANa antisense oligonucleotide suppressed F-LANa expression in human hepatocellular carcinoma BEL-7404 cells and significantly inhibited cell growth. Together, our data demonstrate that overexpression of evolutionarily conserved F-LANa occurs frequently and may play an important role in proliferation.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0006-291X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 Academic Press.
|
| pubmed:issnType |
Print
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| pubmed:day |
17
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| pubmed:volume |
286
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
394-400
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| pubmed:dateRevised |
2006-11-20
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| pubmed:meshHeading |
pubmed-meshheading:11500051-Amino Acid Sequence,
pubmed-meshheading:11500051-Base Sequence,
pubmed-meshheading:11500051-Carcinoma, Hepatocellular,
pubmed-meshheading:11500051-Cell Division,
pubmed-meshheading:11500051-Cloning, Molecular,
pubmed-meshheading:11500051-Gene Expression Profiling,
pubmed-meshheading:11500051-Humans,
pubmed-meshheading:11500051-Liver Neoplasms,
pubmed-meshheading:11500051-Membrane Proteins,
pubmed-meshheading:11500051-Molecular Sequence Data,
pubmed-meshheading:11500051-Neoplasm Proteins,
pubmed-meshheading:11500051-Oligonucleotides, Antisense,
pubmed-meshheading:11500051-Phylogeny,
pubmed-meshheading:11500051-RNA, Neoplasm,
pubmed-meshheading:11500051-Sequence Homology, Amino Acid,
pubmed-meshheading:11500051-Tissue Distribution,
pubmed-meshheading:11500051-Tumor Cells, Cultured,
pubmed-meshheading:11500051-Up-Regulation
|
| pubmed:year |
2001
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| pubmed:articleTitle |
Cloning and characterization of F-LANa, upregulated in human liver cancer.
|
| pubmed:affiliation |
Laboratory of Molecular and Cellular Oncology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai, 200031, People's Republic of China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|