| pubmed-article:11498242 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11498242 | lifeskim:mentions | umls-concept:C0036108 | lld:lifeskim |
| pubmed-article:11498242 | lifeskim:mentions | umls-concept:C0021753 | lld:lifeskim |
| pubmed-article:11498242 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:11498242 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:11498242 | pubmed:dateCreated | 2001-8-10 | lld:pubmed |
| pubmed-article:11498242 | pubmed:abstractText | The predicted rainbow trout mature interleukin-1 beta (IL-1 beta) peptide has been produced as a recombinant protein in E. coli. The bioactivity of this molecule has been studied using trout head kidney cell preparations and a trout macrophage cell line (RTS11). Trout rIL-1 beta was shown to increase the expression level of IL-1 beta, cyclooxygenase (COX2) and MHC class II beta chain transcription, as determined by Northern blot analysis. Stimulatory doses of rIL-1 beta were typically > or =10 ng/ml. Induction of IL-1 beta expression occurred within 1h post-stimulation with trout rIL-1 beta and was maximal 3-6h post-stimulation. Trout rIL-1 beta was also able to increase murine D10.G4.1 cell proliferation and trout head kidney leukocyte phagocytic activity, in a dose-dependent manner. However, equivalent D10.G4.1 cell proliferation was induced with approximately 1000-fold lower doses of human rIL-1 beta. That LPS contamination did not contribute to the effects seen was confirmed by determining its concentration in the trout rIL-1 beta preparation, and demonstrating that the rIL-1 beta activity was inhibited by heating or pre-incubation with a polyclonal anti-trout rIL-1 beta antibody. | lld:pubmed |
| pubmed-article:11498242 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11498242 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11498242 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11498242 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11498242 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11498242 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11498242 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11498242 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:11498242 | pubmed:issn | 0165-2427 | lld:pubmed |
| pubmed-article:11498242 | pubmed:author | pubmed-author:CunninghamCC | lld:pubmed |
| pubmed-article:11498242 | pubmed:author | pubmed-author:BolzAA | lld:pubmed |
| pubmed-article:11498242 | pubmed:author | pubmed-author:HongSS | lld:pubmed |
| pubmed-article:11498242 | pubmed:author | pubmed-author:SecombesC JCJ | lld:pubmed |
| pubmed-article:11498242 | pubmed:author | pubmed-author:ScapigliatiGG | lld:pubmed |
| pubmed-article:11498242 | pubmed:author | pubmed-author:ZouJJ | lld:pubmed |
| pubmed-article:11498242 | pubmed:author | pubmed-author:PeddieSS | lld:pubmed |
| pubmed-article:11498242 | pubmed:author | pubmed-author:CrampeMM | lld:pubmed |
| pubmed-article:11498242 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11498242 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:11498242 | pubmed:volume | 81 | lld:pubmed |
| pubmed-article:11498242 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11498242 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11498242 | pubmed:pagination | 1-14 | lld:pubmed |
| pubmed-article:11498242 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11498242 | pubmed:meshHeading | pubmed-meshheading:11498242... | lld:pubmed |
| pubmed-article:11498242 | pubmed:meshHeading | pubmed-meshheading:11498242... | lld:pubmed |
| pubmed-article:11498242 | pubmed:meshHeading | pubmed-meshheading:11498242... | lld:pubmed |
| pubmed-article:11498242 | pubmed:meshHeading | pubmed-meshheading:11498242... | lld:pubmed |
| pubmed-article:11498242 | pubmed:meshHeading | pubmed-meshheading:11498242... | lld:pubmed |
| pubmed-article:11498242 | pubmed:meshHeading | pubmed-meshheading:11498242... | lld:pubmed |
| pubmed-article:11498242 | pubmed:meshHeading | pubmed-meshheading:11498242... | lld:pubmed |
| pubmed-article:11498242 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11498242 | pubmed:articleTitle | The production and bioactivity of rainbow trout (Oncorhynchus mykiss) recombinant IL-1 beta. | lld:pubmed |
| pubmed-article:11498242 | pubmed:affiliation | Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen AB24 2TZ, Scotland, UK. | lld:pubmed |
| pubmed-article:11498242 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11498242 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11498242 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11498242 | lld:pubmed |
