Source:http://linkedlifedata.com/resource/pubmed/id/11490008
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2001-8-7
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| pubmed:abstractText |
We examined TCR usage to a protective fragment of heat shock protein 60 from the fungus, Histoplasma capsulatum. Nearly 90% of T cell clones from C57BL/6 mice vaccinated with this protein were Vbeta6+; the remainder were Vbeta14+. Amino acid motifs of the CDR3 region from Vbeta6+ cells were predominantly IxGGG, IGG, or SxxGG, whereas it was uniformly SFSGG for Vbeta14+ clones. Short term T cell lines from Vbeta6+-depleted mice failed to recognize Ag, and no T cell clones could be generated. To determine whether Vbeta6+ cells were functionally important, we eliminated them during vaccination. Depletion of Vbeta6+ cells abrogated protection in vivo and upon adoptive transfer of cells into TCR alphabeta(-/-) mice. Transfer of a Vbeta6+, but not a Vbeta14+, clone into TCR alphabeta(-/-) mice prolonged survival. Cytokine generation by Ag-stimulated splenocytes from immunized mice depleted of Vbeta6+ cells was similar to that of controls. The efficacy of the Vbeta6+ clone was associated with elevated production of IFN-gamma, TNF-alpha, and GM-CSF compared with that of the Vbeta14+ clone. More Vbeta6+ cells were present in lungs and spleens of TCR alphabeta(-/-) on day 3 postinfection compared with Vbeta14+ cells. Thus, a single Vbeta family was essential for vaccine-induced immunity. Moreover, the mechanism by which Vbeta6+ contributed to protective immunity differed between unfractionated splenocytes and T cell clones.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chaperonin 60,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
167
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2219-26
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11490008-Adoptive Transfer,
pubmed-meshheading:11490008-Animals,
pubmed-meshheading:11490008-Base Sequence,
pubmed-meshheading:11490008-Cell Line,
pubmed-meshheading:11490008-Chaperonin 60,
pubmed-meshheading:11490008-Clone Cells,
pubmed-meshheading:11490008-Cytokines,
pubmed-meshheading:11490008-Fungal Vaccines,
pubmed-meshheading:11490008-Histoplasma,
pubmed-meshheading:11490008-Histoplasmosis,
pubmed-meshheading:11490008-Injections, Intravenous,
pubmed-meshheading:11490008-Injections, Subcutaneous,
pubmed-meshheading:11490008-Lymphocyte Depletion,
pubmed-meshheading:11490008-Mice,
pubmed-meshheading:11490008-Mice, Inbred C57BL,
pubmed-meshheading:11490008-Mice, Knockout,
pubmed-meshheading:11490008-Peptide Fragments,
pubmed-meshheading:11490008-Protein Structure, Tertiary,
pubmed-meshheading:11490008-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:11490008-Spleen,
pubmed-meshheading:11490008-T-Lymphocyte Subsets
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| pubmed:year |
2001
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| pubmed:articleTitle |
V beta 6+ T cells are obligatory for vaccine-induced immunity to Histoplasma capsulatum.
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| pubmed:affiliation |
Division of Infectious Diseases, University of Cincinnati College of Medicine, and Veterans Affairs Hospital, Cincinnati, OH 45267, USA. george.deepe@uc.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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