Linked Life Data

11485973

Source:http://linkedlifedata.com/resource/pubmed/id/11485973

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-8-3
pubmed:abstractText
Telomeres are primarily controlled by a highly specialized DNA polymerase termed telomerase. Recent studies have demonstrated that introduction of the telomerase catalytic component (TERT) into telomerase-negative cells activates telomerase and extends cell life span, whereas mice lacking telomerase activity revealed impaired cell proliferation in some organs as well as reduced tumorigenesis. These reports suggest that telomerase plays an important role in long-term cell viability and cell proliferation. However, the mechanism or mechanisms by which telomerase is induced or regulated remains to be elucidated. We report here that primary vascular smooth muscle cells (VSMCs) express telomerase and that increased telomerase activity correlates with cell proliferation. Inhibition of telomerase diminished growth of VSMCs, which suggests a crucial role for telomerase activation in the regulation of VSMC proliferation. We propose a novel model whereby telomerase is first activated in the cytoplasm before cell proliferation, followed by accumulation of activity in the nucleus during the logarithmic phase of cell growth. Activation of telomerase in VSMCs was linked to phosphorylation of TERT. The protein kinase inhibitor H7 suppressed the activation of telomerase in the cytoplasm and also inhibited the accumulation of TERT as well as telomerase activity in the nucleus. These data suggest that posttranslational modification of TERT by phosphorylation is important for activation and accumulation of telomerase into the nucleus in the process of VSMC proliferation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1524-4571
pubmed:author
pubmed:issnType
Electronic
pubmed:day
3
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-43
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11485973-Adenosine Triphosphate, pubmed-meshheading:11485973-Animals, pubmed-meshheading:11485973-Blotting, Western, pubmed-meshheading:11485973-Carrier Proteins, pubmed-meshheading:11485973-Cell Division, pubmed-meshheading:11485973-Cell Nucleus, pubmed-meshheading:11485973-Cells, Cultured, pubmed-meshheading:11485973-Cytoplasm, pubmed-meshheading:11485973-DNA-Binding Proteins, pubmed-meshheading:11485973-Enzyme Activation, pubmed-meshheading:11485973-Enzyme Induction, pubmed-meshheading:11485973-Enzyme Inhibitors, pubmed-meshheading:11485973-Muscle, Smooth, Vascular, pubmed-meshheading:11485973-Phosphorylation, pubmed-meshheading:11485973-Polymerase Chain Reaction, pubmed-meshheading:11485973-Protein Kinase Inhibitors, pubmed-meshheading:11485973-RNA, pubmed-meshheading:11485973-RNA, Messenger, pubmed-meshheading:11485973-Rats, pubmed-meshheading:11485973-Telomerase
pubmed:year
2001
pubmed:articleTitle
Mechanisms of telomerase induction during vascular smooth muscle cell proliferation.
pubmed:affiliation
Department of Medicine, Division of Newborn Medicine, Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't