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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-7-5
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pubmed:abstractText |
The specific aminoacylation of tRNA is critical for translation of the genetic code. A molecular description of aminoacylation requires knowledge of the relevant three-dimensional structures, biochemical parameters and the structure-function relationship of the synthetase and its substrate tRNA. Extensive structural and biochemical data are available on the aspartic acid system of Escherichia coli, but there is a paucity of cellular functional data. We have developed a system to overcome this deficiency by engineering an E. coli knockout tRNA(Asp) strain, thereby allowing a penetrating analysis of tRNA(Asp) structure and function under conditions that prevail in the cell.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-2836
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
310
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
537-42
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11439021-Acylation,
pubmed-meshheading:11439021-Anticodon,
pubmed-meshheading:11439021-Aspartate-tRNA Ligase,
pubmed-meshheading:11439021-Aspartic Acid,
pubmed-meshheading:11439021-Base Sequence,
pubmed-meshheading:11439021-Blotting, Northern,
pubmed-meshheading:11439021-Crystallography, X-Ray,
pubmed-meshheading:11439021-Escherichia coli,
pubmed-meshheading:11439021-Gene Deletion,
pubmed-meshheading:11439021-Genes, Bacterial,
pubmed-meshheading:11439021-Molecular Sequence Data,
pubmed-meshheading:11439021-Nucleic Acid Conformation,
pubmed-meshheading:11439021-Plasmids,
pubmed-meshheading:11439021-RNA, Bacterial,
pubmed-meshheading:11439021-RNA, Transfer, Asp,
pubmed-meshheading:11439021-Structure-Activity Relationship
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pubmed:year |
2001
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pubmed:articleTitle |
Construction of an Escherichia coli knockout strain for functional analysis of tRNA(Asp).
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pubmed:affiliation |
Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, E. B. Fred Hall, Madison, WI 53706-1567, USA. wmcclain@facstaff.wisc.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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