Source:http://linkedlifedata.com/resource/pubmed/id/11406647
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2001-6-14
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| pubmed:abstractText |
SUMMARY: DOC-2/hDab2 (DOC-2) has tumor suppressive functions in ovarian cancer and choriocarcinoma. In these tumors, it negatively influences mitogenic signal transduction of growth factors and blocks ras activity. Pancreatic cancer exhibits a high frequency of K-ras gene mutations; however, it is not known whether DOC-2 expression is altered in these tumors. Therefore, we investigated DOC-2 expression in 22 pancreatic adenocarcinomas and in 6 pancreatic cancer cell lines. Findings in human tumors were compared with normal controls and correlated with clinicopathological data. Additionally, the influence of K-ras on DOC-2 transcription was investigated. Northern blot and Western blot analyses both demonstrated an increase of DOC-2 mRNA and protein levels in primary pancreatic cancers in comparison with normal controls. In situ hybridization showed DOC-2 mRNA expression in the majority of cancer cells of primary tumors, as well as in chronic pancreatitis-like lesions surrounding the cancer mass. Immunohistochemistry mirrored the in situ hybridization findings. In contrast, levels of expression of DOC-2 in lymph node metastases were markedly decreased in comparison with levels in primary tumors. In addition, in 5 metastatic pancreatic cancer cell lines, DOC-2 mRNA and protein levels were low, whereas quantitative RT-PCR demonstrated relatively higher levels in a nonmetastatic pancreatic cancer cell line. In conclusion, DOC-2 is overexpressed in primary pancreatic adenocarcinoma but down-regulated in metastatic disease, suggesting a tumor suppressor function of DOC-2 in the late steps of pancreatic carcinogenesis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Vesicular...,
http://linkedlifedata.com/resource/pubmed/chemical/DAB2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0023-6837
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
81
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
863-73
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11406647-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:11406647-Adaptor Proteins, Vesicular Transport,
pubmed-meshheading:11406647-Adenocarcinoma,
pubmed-meshheading:11406647-Adult,
pubmed-meshheading:11406647-Aged,
pubmed-meshheading:11406647-Blotting, Northern,
pubmed-meshheading:11406647-Blotting, Western,
pubmed-meshheading:11406647-Female,
pubmed-meshheading:11406647-Genes, Tumor Suppressor,
pubmed-meshheading:11406647-Humans,
pubmed-meshheading:11406647-Immunohistochemistry,
pubmed-meshheading:11406647-In Situ Hybridization,
pubmed-meshheading:11406647-Male,
pubmed-meshheading:11406647-Middle Aged,
pubmed-meshheading:11406647-Pancreas,
pubmed-meshheading:11406647-Pancreatic Neoplasms,
pubmed-meshheading:11406647-Proteins,
pubmed-meshheading:11406647-RNA, Messenger,
pubmed-meshheading:11406647-Reference Values,
pubmed-meshheading:11406647-Tumor Cells, Cultured,
pubmed-meshheading:11406647-Up-Regulation
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| pubmed:year |
2001
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| pubmed:articleTitle |
Doc-2/hDab2 expression is up-regulated in primary pancreatic cancer but reduced in metastasis.
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| pubmed:affiliation |
Department of Visceral and Transplantation Surgery, University of Bern, Inselspital, Bern, Switzerland. helmut.friess@insel.ch
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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