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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2001-5-22
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pubmed:abstractText |
Major histocompatibility complex (MHC) class I-restricted CD8(+) T cells play a critical role in the protective immunity against Mycobacterium tuberculosis (Mtb). However, only a few Mtb peptides recognized by MHC class Ia-restricted CD8(+) T cells have been identified. Information on epitopes recognized by class Ib-restricted T cells is even more limited. M3 is an MHC class Ib molecule that preferentially presents N-formylated peptides to CD8(+) T cells. Because bacteria initiate protein synthesis with N-formyl methionine, the unique binding specificity of M3 makes it especially suitable for presenting these particular bacterial epitopes. We have scanned the full sequence of the Mtb genome for NH2-terminal peptides that share features with other M3-binding peptides. Synthetic peptides corresponding to these sequences were tested for their ability to bind to M3 in an immunofluorescence-based peptide-binding assay. Four of the N-formylated Mtb peptides were able to elicit cytotoxic T lymphocytes (CTLs) from mice immunized with peptide-coated splenocytes. The Mtb peptide-specific, M3-restricted CTLs lysed the Mtb-infected macrophages effectively, suggesting that these N-formylated Mtb peptides are presented as the naturally processed epitopes by Mtb-infected cells. Furthermore, T cells from Mtb-infected lungs, spleen, and lymph nodes responded to N-formylated Mtb peptides in an M3-restricted manner. Taken together, our data suggest that M3-restricted T cells may participate in the immune response to Mtb.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-10377097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-10417164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-10430630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-10432283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-10601361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-10611360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-11369794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-1465432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-1855254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-1898911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-1919442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-2025413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-2450033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-6421492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-7504064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-7527500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-7542404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-7664344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-7686932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-8245795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-8592468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-8671623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-8758895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-8758896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-8876215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9143709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9160097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9174606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9180075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9418128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9419365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9488151,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9634230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9725236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9730898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9770513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11369792-9886409
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
193
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1213-20
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:11369792-Animals,
pubmed-meshheading:11369792-Antigens, Bacterial,
pubmed-meshheading:11369792-Genome, Bacterial,
pubmed-meshheading:11369792-Histocompatibility Antigens Class I,
pubmed-meshheading:11369792-Interferon-gamma,
pubmed-meshheading:11369792-Macrophages,
pubmed-meshheading:11369792-Mice,
pubmed-meshheading:11369792-Mycobacterium tuberculosis,
pubmed-meshheading:11369792-N-Formylmethionine,
pubmed-meshheading:11369792-Oligopeptides,
pubmed-meshheading:11369792-Phenotype,
pubmed-meshheading:11369792-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:11369792-Tuberculosis
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pubmed:year |
2001
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pubmed:articleTitle |
Induction of M3-restricted cytotoxic T lymphocyte responses by N-formylated peptides derived from Mycobacterium tuberculosis.
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pubmed:affiliation |
Gwen Knapp Center for Lupus and Immunology Research, Committee on Immunology and Department of Pathology, University of Chicago, Chicago, Illinois 60637, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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