Linked Life Data

11358180

Source:http://linkedlifedata.com/resource/pubmed/id/11358180

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-5-18
pubmed:abstractText
A combination of three plates, seeded with strains of Micrococcus luteus, Bacillus cereus or Escherichia coli, can be used for detection of residues of beta-lactam antibiotics, tetracyclines and fluoroquinolones. The sensitivity of each plate is optimal for only one of these groups, resulting in detection limits (LOD) lower than the corresponding maximum residue limits (MRL) and in distinct inhibition patterns typical for each antibiotic family. Beta-lactam antibiotics such as penicillin G, ampicillin and amoxicillin give only inhibition zones on the plate with M. luteus. Tetracyclines are detected up to the MRL level with B. cereus, and fluoroquinolones with E. coli. The LODs of the antibiotics tested were as follows: penicillin G (PENG) 0.9 ng, ampicillin (AMPI) 0.6 ng and amoxicillin (AMOX) 1.0 ng on the plate with M. luteus; tetracycline (TET) 4 ng, oxytetracycline (OXY) 3 ng, doxycycline (DOX) 0.6 ng, and chlortetracycline (CHL) 0.3 ng on the plate with B. cereus; enrofloxacin (ENRX) 1.5 ng, ciprofloxacin (CIPX) 0.5 ng and flumequine (FLUM) 1.5 ng on the plate with E. coli. The combination of plates enables the laboratory to select appropriate chromatographic techniques for identification and quantification of the residues. On the other hand, the three groups can also be detected on one plate seeded with Bacillus subtilis, although the limits of detection are higher: PENG 0.4 ng, AMPI and AMOX 3 ng, TET 5 ng, OXY 8 ng, DOX 1 ng, CHL 0.5 ng, ENRX 4 ng, CIPX 10 ng and FLUM 4 ng. The test was applied to 228 broiler fillets and to 27 turkey thighs, originating from different poultry slaughterhouses. Nineteen broiler fillets contained inhibiting substances. The positive results of the inhibition tests were confirmed with a chromatographic technique. Doxycycline residues were found in 16 samples and amoxicillin in two.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0265-203X
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
385-93
pubmed:dateRevised
2004-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Inhibition tests for detection and presumptive identification of tetracyclines, beta-lactam antibiotics and quinolones in poultry meat.
pubmed:affiliation
Department of Veterinary Food Inspection, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. godelieve.okerman@rug.ac.be
pubmed:publicationType
Journal Article, Evaluation Studies