Linked Life Data

11331516

Source:http://linkedlifedata.com/resource/pubmed/id/11331516

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-5-1
pubmed:abstractText
We modify and apply a nonlethal technique for rapidly quantifying the cold tolerance of large numbers of Drosophila and other small insects. Flies are transferred to individual vials, cooled in groups in progressive 0.5 degrees C steps, and checked for loss of righting response (chill-coma temperature [T(cc)]). Flies recover quickly when transferred to ambient temperature, and thus this technique potentially can be used in selection experiments. We applied this technique in several experiments. First, we examined the sensitivity of T(cc) to developmental temperature. Drosophila melanogaster (Congo, France), Drosophila subobscura (Spain, Denmark), and Drosophila ananassae (India) were reared from egg to adult at 15 degrees, 18 degrees, 25 degrees, or 29 degrees C, transferred to 15 degrees C for several days, and then progressively chilled: T(cc) was positively related to developmental temperature, inversely related to latitude of the population, but independent of sex. The sensitivity of T(cc) to developmental temperature (acclimation flexibility) was marked: T(cc) shifted on average 1 degrees for each 4 degrees C shift in developmental temperature. Among 15 species of the obscura group of Drosophila, T(cc) varied from -0.1 degrees to 4.5 degrees C; T(cc) was inversely related to latitude in both nonphylogenetic and phylogenetically based ANCOVA (standardized independent contrasts) and was unrelated to body size.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1522-2152
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
429-34
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
Chill-coma temperature in Drosophila: effects of developmental temperature, latitude, and phylogeny.
pubmed:affiliation
Laboratoire Populations, Genetique, et Evolution, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette Cedex, France. Patricia.Gilbert@pge.cnrs-gif.fr
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't