Linked Life Data

11329458

Source:http://linkedlifedata.com/resource/pubmed/id/11329458

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-5-1
pubmed:abstractText
Normal assay variation associated with bDNA tests for human immunodeficiency virus type 1 (HIV-1) RNA performed at two laboratories with different levels of test experience was investigated. Two 5-ml aliquots of blood in EDTA tubes were collected from each patient for whom the HIV-1 bDNA test was ordered. Blood was stored for no more than 4 h at room temperature prior to plasma separation. Plasma was stored at -70 degrees C until transported to the Central Pennsylvania Alliance Laboratory (CPAL; York, Pa.) and to the Hershey Medical Center (Hershey, Pa.) on dry ice. Samples were stored at < or =-70 degrees C at both laboratories prior to testing. Pools of negative (donor), low-HIV-1-RNA-positive, and high-HIV-1-RNA-positive plasma samples were also repeatedly tested at CPAL to determine both intra- and interrun variation. From 11 August 1999 until 14 September 2000, 448 patient specimens were analyzed in parallel at CPAL and Hershey. From 206 samples with results of > or =1,000 copies/ml at CPAL, 148 (72%) of the results varied by < or =0.20 log(10) when tested at Hershey and none varied by >0.50 log(10). However, of 242 specimens with results of <1,000 copies/ml at CPAL, 11 (5%) of the results varied by >0.50 log(10) when tested at Hershey. Of 38 aliquots of HIV-1 RNA pool negative samples included in 13 CPAL bDNA runs, 37 (97%) gave results of <50 copies/ml and 1 (3%) gave a result of 114 copies/ml. Low-positive HIV-1 RNA pool intrarun variation ranged from 0.06 to 0.26 log(10) while the maximum interrun variation was 0.52 log(10). High-positive HIV-1 RNA pool intrarun variation ranged from 0.04 to 0.32 log(10), while the maximum interrun variation was 0.55 log(10). In our patient population, a change in bDNA HIV-1 RNA results of < or =0.50 log(10) over time most likely represents normal laboratory test variation. However, a change of >0.50 log(10), especially if the results are >1,000 copies/ml, is likely to be significant.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-10523562, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-10699005, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-10921936, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-11060065, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-7650187, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-8370724, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-8897167, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-8940441, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-9242803, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-9508301, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-9537934, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-9542939, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-9620364, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-9646000, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329458-9890848
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
560-3
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Intra- and interlaboratory variabilities of results obtained with the Quantiplex human immunodeficiency virus type 1 RNA bDNA assay, version 3.0.
pubmed:affiliation
Central Pennsylvania Alliance Laboratory, York, Pennsylvania, USA. jkellogg@wellspan.org
pubmed:publicationType
Journal Article