Linked Life Data

11220773

Source:http://linkedlifedata.com/resource/pubmed/id/11220773

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-2-23
pubmed:abstractText
We have characterized brain cytokine expression profiles in the Plasmodium coatneyi/rhesus (Macaque mulatta) malaria model. Eight rhesus monkeys were included in the study; four were infected with P. coatneyi, and four were used as uninfected controls. All inoculated animals became infected. Eleven days after parasite inoculation, the rhesus monkeys were killed and tissue samples from 4 regions of the brain (cortex and white matter of the cerebrum, cerebellum, and midbrain) were collected for quantitation of mRNA expression of cytokines, adhesion molecules, and inducible nitric oxide synthetase (iNOS) by reverse transcriptase-polymerase chain reaction (RT-PCR). The expression levels of tumor necrosis actor-alpha (TNF-alpha), gamma interferon (IFN-gamma), interleukin-1-beta (IL-1beta), intercellular adhesion molecule-1 (ICAM-1) and inducible nitric oxide synethetase (iNOS) were highest in the cerebellum of infected animals, correlating well with pathologic observations of sequestration of parasitized erythrocytes in this region of the brain. Infected animals also had higher TNF-alpha expression levels in the cortex and IL-1beta expression levels in the cortex, white matter, and midbrain. Thus, the expression of pro-inflammatory and T helper-1 (TH-1) cytokines, adhesion molecules, and iNOS appears to predominate in the cerebellum of infected rhesus monkeys.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0002-9637
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
530-4
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11220773-Animals, pubmed-meshheading:11220773-Brain, pubmed-meshheading:11220773-Cerebellum, pubmed-meshheading:11220773-Cerebral Cortex, pubmed-meshheading:11220773-Cytokines, pubmed-meshheading:11220773-Disease Models, Animal, pubmed-meshheading:11220773-Gene Expression, pubmed-meshheading:11220773-Intercellular Adhesion Molecule-1, pubmed-meshheading:11220773-Interferon-gamma, pubmed-meshheading:11220773-Interleukin-1, pubmed-meshheading:11220773-Interleukin-12, pubmed-meshheading:11220773-Macaca mulatta, pubmed-meshheading:11220773-Malaria, pubmed-meshheading:11220773-Mesencephalon, pubmed-meshheading:11220773-Microcirculation, pubmed-meshheading:11220773-Nitric Oxide Synthase, pubmed-meshheading:11220773-Nitric Oxide Synthase Type II, pubmed-meshheading:11220773-Parasitemia, pubmed-meshheading:11220773-RNA, Messenger, pubmed-meshheading:11220773-Telencephalon, pubmed-meshheading:11220773-Tumor Necrosis Factor-alpha
pubmed:year
2000
pubmed:articleTitle
Expression of proinflammatory cytokines in four regions of the brain in Macaque mulatta (rhesus) monkeys infected with Plasmodium coatneyi.
pubmed:affiliation
National Center for Infectious Diseases, Centers for Disease Control and Prevention, US Department of Health and Human Services, Atlanta, Georgia 30341, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't