Linked Life Data

11216863

Source:http://linkedlifedata.com/resource/pubmed/id/11216863

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2001-2-16
pubmed:abstractText
Leishmania donovani, an obligate intracellular parasite resides and multiplies within macrophage of the reticuloendothelial system. The intracellular signalling mechanism involved in the impaired oxidative response in leishmaniasis has not yet been clearly established. Generation of superoxide anion (O2-) is supposed to be the first line of host defence during microbial invasion. We found a substantial inhibition of superoxide anion generation in parasitized macrophages, which was just the reverse in case of macrophages challenged with Lipophosphoglycan (LPG) deficient attenuated leishmanial parasite UR-6. The generation of O2- essentially needs the prior activation of protein kinase C (PKC) mediated phosphorylation events. Our study proposed that phosphorylation of 67, 54, 47 and 36 kDa proteins was attenuated during infection. This was supported by PKC activity study, where Ca-dependent PKC activity was inhibited but, Ca-independent PKC activity was enhanced. This result was further confirmed by using isotype specific pseudosubstrate inhibitors of Ca-dependent PKC beta and Ca-independent PKC zeta. Application of beta-pseudosubstrate could not alter the Ca-dependent PKC activity but zeta-pseudosubstrate inhibited the Ca-independent PKC activity in infected macrophages. Our immunoblot analysis with specific antibody against PKC beta and PKC zeta isotypes showed down regulation of PKC beta-II expression with concomitant induction of PKC zeta. Such inhibition of Ca-dependent PKC beta was reversed in macrophages treated with UR-6. Taken together, our observations revealed that infection with L. donovani selectively attenuates both the expression and activity of Ca-dependent PKC beta.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0300-8177
pubmed:author
pubmed:issnType
Print
pubmed:volume
216
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-57
pubmed:dateRevised
2009-4-7
pubmed:meshHeading
pubmed-meshheading:11216863-Animals, pubmed-meshheading:11216863-Blotting, Northern, pubmed-meshheading:11216863-Calcium, pubmed-meshheading:11216863-Cell Adhesion, pubmed-meshheading:11216863-Densitometry, pubmed-meshheading:11216863-Down-Regulation, pubmed-meshheading:11216863-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:11216863-Enzyme Activation, pubmed-meshheading:11216863-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:11216863-Female, pubmed-meshheading:11216863-Glycosphingolipids, pubmed-meshheading:11216863-Immunoblotting, pubmed-meshheading:11216863-Leishmania donovani, pubmed-meshheading:11216863-Macrophages, pubmed-meshheading:11216863-Male, pubmed-meshheading:11216863-Mice, pubmed-meshheading:11216863-Mice, Inbred BALB C, pubmed-meshheading:11216863-Peritoneum, pubmed-meshheading:11216863-Phosphorylation, pubmed-meshheading:11216863-Protein Kinase C, pubmed-meshheading:11216863-Signal Transduction, pubmed-meshheading:11216863-Superoxides, pubmed-meshheading:11216863-Time Factors, pubmed-meshheading:11216863-Tumor Necrosis Factor-alpha
pubmed:year
2001
pubmed:articleTitle
Selective impairment of protein kinase C isotypes in murine macrophage by Leishmania donovani.
pubmed:affiliation
Department of Microbiology, Bose Institute, Calcutta, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't