| pubmed-article:11163396 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11163396 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11163396 | lifeskim:mentions | umls-concept:C0682323 | lld:lifeskim |
| pubmed-article:11163396 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:11163396 | lifeskim:mentions | umls-concept:C0001239 | lld:lifeskim |
| pubmed-article:11163396 | lifeskim:mentions | umls-concept:C1414635 | lld:lifeskim |
| pubmed-article:11163396 | lifeskim:mentions | umls-concept:C1135629 | lld:lifeskim |
| pubmed-article:11163396 | lifeskim:mentions | umls-concept:C1414637 | lld:lifeskim |
| pubmed-article:11163396 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:11163396 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:11163396 | pubmed:dateCreated | 2001-2-22 | lld:pubmed |
| pubmed-article:11163396 | pubmed:abstractText | Human Lnk (hLnk) is an adaptor protein with multiple functional domains that regulates T cell activation signaling. In order to identify cellular Lnk binding partners, a yeast two-hybrid screening of human spleen cDNA library was carried out using human hLnk as bait. A polypeptide sequence identical to the C-terminal segment of the actin binding protein (ABP-280) was identified as a hLnk binding protein. The expressed hLnk and the FLAG tagged C-terminal 673 amino acid residues of ABP-280 or the endogenous ABP-280 in COS-7 cells could be co-immunoprecipitated using antibodies either to hLnk, FLAG or ABP-280, respectively. Furthermore, immunofluorescence confocal microscope showed that hLnk and ABP-280 co-localized at the plasma membrane and at juxtanuclear region of COS-7 cells. In Jurkat cells, the endogenous hLnk also associates with the endogenous ABP-280 indicating that the association of these two proteins is physiological. The interacting domains of both proteins were mapped using yeast two-hybrid assays. Our results indicate that hLnk binds to the residues 2006-2454 (repeats 19-23C) of ABP-280. The domain in hLnk that associates with ABP-280 was mapped to an interdomain region of 56 amino acids between pleckstrin homology and Src homology 2 domains. These results suggest that hLnk may exert its regulatory role through its association with ABP-280. | lld:pubmed |
| pubmed-article:11163396 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11163396 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11163396 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11163396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11163396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11163396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11163396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11163396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11163396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11163396 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11163396 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:11163396 | pubmed:issn | 0161-5890 | lld:pubmed |
| pubmed-article:11163396 | pubmed:author | pubmed-author:LUMM | lld:pubmed |
| pubmed-article:11163396 | pubmed:author | pubmed-author:HayashiJJ | lld:pubmed |
| pubmed-article:11163396 | pubmed:author | pubmed-author:HUHH | lld:pubmed |
| pubmed-article:11163396 | pubmed:author | pubmed-author:Schembri-King... | lld:pubmed |
| pubmed-article:11163396 | pubmed:author | pubmed-author:JakesSS | lld:pubmed |
| pubmed-article:11163396 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11163396 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:11163396 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11163396 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11163396 | pubmed:pagination | 603-12 | lld:pubmed |
| pubmed-article:11163396 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11163396 | pubmed:meshHeading | pubmed-meshheading:11163396... | lld:pubmed |
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| pubmed-article:11163396 | pubmed:meshHeading | pubmed-meshheading:11163396... | lld:pubmed |
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| pubmed-article:11163396 | pubmed:meshHeading | pubmed-meshheading:11163396... | lld:pubmed |
| pubmed-article:11163396 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11163396 | pubmed:articleTitle | Identification of actin binding protein, ABP-280, as a binding partner of human Lnk adaptor protein. | lld:pubmed |
| pubmed-article:11163396 | pubmed:affiliation | School of Pharmacy, University of Maryland, 20 North Pine Street, Baltimore, MD 21201, USA. | lld:pubmed |
| pubmed-article:11163396 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11163396 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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