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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-1-3
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pubmed:databankReference |
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pubmed:abstractText |
Persistent immunity against Leishmania: infections in humans is mediated predominantly by CD4(+) T cells of the Th1 phenotype. Herein we report the expression cloning of eight Leishmania: Ags using parasite-specific T cell lines derived from an immune donor. The Ags identified by this technique include the flagellar proteins alpha- and beta-tubulin, histone H2b, ribosomal protein S4, malate dehydrogenase, and elongation factor 2, as well as two novel parasite proteins. None of these proteins have been previously reported as T cell-stimulating Ags from Leishmania: beta-tubulin-specific T cell clones generated against Leishmania: major amastigotes responded to Leishmania:-infected macrophages and dendritic cells. IFN-gamma enzyme-linked immunospot analysis demonstrated the presence of T cells specific for several of these Ags in PBMC from self-healing cutaneous leishmaniasis patients infected with either Leishmania: tropica or L. major. The responses elicited by Leishmania: histone H2b were particularly striking in terms of frequency of histone-specific T cells in PBMC (1 T cell of 6000 PBMC) as well as the percentage of responding donors (86%, 6 of 7). Ags identified by T cells from immune donors might constitute potential vaccine candidates for leishmaniasis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
166
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
498-505
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11123329-Animals,
pubmed-meshheading:11123329-Antigens, Protozoan,
pubmed-meshheading:11123329-Bacteriophage lambda,
pubmed-meshheading:11123329-CD4-Positive T-Lymphocytes,
pubmed-meshheading:11123329-Cell Line,
pubmed-meshheading:11123329-Clone Cells,
pubmed-meshheading:11123329-Cloning, Molecular,
pubmed-meshheading:11123329-Dendritic Cells,
pubmed-meshheading:11123329-Epitopes, T-Lymphocyte,
pubmed-meshheading:11123329-Gene Library,
pubmed-meshheading:11123329-Histones,
pubmed-meshheading:11123329-Humans,
pubmed-meshheading:11123329-Leishmania major,
pubmed-meshheading:11123329-Leishmania tropica,
pubmed-meshheading:11123329-Leishmaniasis, Cutaneous,
pubmed-meshheading:11123329-Leukocytes, Mononuclear,
pubmed-meshheading:11123329-Lymphocyte Activation,
pubmed-meshheading:11123329-Macrophages,
pubmed-meshheading:11123329-Malate Dehydrogenase,
pubmed-meshheading:11123329-Male,
pubmed-meshheading:11123329-Molecular Sequence Data,
pubmed-meshheading:11123329-Recombinant Fusion Proteins,
pubmed-meshheading:11123329-T-Lymphocyte Subsets,
pubmed-meshheading:11123329-Tubulin
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pubmed:year |
2001
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pubmed:articleTitle |
Identification and characterization of T cell-stimulating antigens from Leishmania by CD4 T cell expression cloning.
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pubmed:affiliation |
Corixa Corporation, Seattle, WA 98104, USA. jwebb@uottawa.ca
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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