Linked Life Data

11104508

Source:http://linkedlifedata.com/resource/pubmed/id/11104508

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-12-20
pubmed:abstractText
Using insulin-like growth factor-I (IGF-I)-overexpressing transgenic (Tg) mice as a model, we have shown that IGF-I promotes myelination by increasing the number of oligodendrocytes and stimulating the expression of myelin-specific protein genes. In the present study, we investigated whether IGF-I protects myelination from undernutritional insult in Tg mice. Mice were undernourished beginning from postnatal (P) day 1, a time coincident with the onset of transgene expression, and sacrificed at P20. Consistently with our previous studies, brain weights of undernourished non-Tg control mice were decreased by approximately 18%. Brain weights of undernourished IGF-I Tg mice, however, were the same as those of well-fed control mice and much greater than those of undernourished control mice. The expression of two major myelin proteins [myelin basic protein (MBP) and proteolipid protein (PLP)] in cerebral cortex (CTX) and hippocampus (HIP) was decreased by 73-92% in undernourished control mice, as judged by Northern and Western blot hybridization. The abundances of MBP and PLP mRNAs and proteins, however, were decreased by only 40-70% in undernourished IGF-I Tg mice. To assess the number of oligodendrocytes and their precursors, antibodies specific for carbonic anhydrase II (CAII; an oligodendrocyte marker) and NG2 (a precursor marker) were used. Compared to their well-fed counterparts, undernourished control mice exhibited 17-30% decreases in the number of oligodendrocytes and their precursors in CTX and corpus callosum (CC), whereas well-fed IGF-I Tg mice had 21-35% increases in CTX and CC. Undernourished IGF-I Tg mice exhibited cell numbers similar to those of well-fed control mice. These data indicate that IGF-I protects myelination from undernutrition damage during development.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
700-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11104508-Animals, pubmed-meshheading:11104508-Animals, Newborn, pubmed-meshheading:11104508-Blotting, Northern, pubmed-meshheading:11104508-Blotting, Western, pubmed-meshheading:11104508-Brain, pubmed-meshheading:11104508-Cell Count, pubmed-meshheading:11104508-Cerebral Cortex, pubmed-meshheading:11104508-Corpus Callosum, pubmed-meshheading:11104508-Food Deprivation, pubmed-meshheading:11104508-Immunohistochemistry, pubmed-meshheading:11104508-Insulin-Like Growth Factor I, pubmed-meshheading:11104508-Mice, pubmed-meshheading:11104508-Mice, Transgenic, pubmed-meshheading:11104508-Myelin Basic Proteins, pubmed-meshheading:11104508-Myelin Proteins, pubmed-meshheading:11104508-Myelin Proteolipid Protein, pubmed-meshheading:11104508-Oligodendroglia, pubmed-meshheading:11104508-RNA, Messenger
pubmed:year
2000
pubmed:articleTitle
Insulin-like growth factor-I (IGF-I) protects myelination from undernutritional insult: studies of transgenic mice overexpressing IGF-I in brain.
pubmed:affiliation
Department of Pediatrics, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7220, USA. Ping_Ye@med.unc.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.