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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-10-12
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pubmed:abstractText |
We investigated the roles of beta(1)- and beta(2)-receptors (beta-AR) in adrenergic enhancement of L-type Ca(2+) current (I(CaL)) in canine ventricular myocytes. Isoproterenol and l-norepinephrine produced a monophasic and a biphasic concentration-I(CaL) relationship (CR), respectively. alpha(1)-AR inhibition with prazosin and beta(2)-AR stimulation with zinterol or l-epinephrine shifted the CR of l-norepinephrine leftward. Zinterol (50 nM) and l-epinephrine (10 nM), but not prazosin, altered the biphasic CR of l-norepinephrine to a monophasic CR. Zinterol and l-epinephrine applied after l-norepinephrine had no effect on I(CaL). beta(2)-AR inhibition with ICI-118551 reduced the E(max) of isoproterenol and l-norepinephrine by 60% and abolished the augmentation of l-norepinephrine by zinterol and l-epinephrine. Carbachol (100 nM) modestly reduced the I(CaL) response to beta(1)-AR stimulation but abolished the enhancement via beta(2)-AR. Zinterol augmented the enhancement of I(CaL) by forskolin, IBMX, and theophylline, but not in the presence of CGP-20712A. We conclude that selective beta(2)-AR stimulation does not increase I(CaL) but enhances adenylyl cyclase activity when stimulated via beta(1)-AR and with forskolin. beta(2)-AR activity preconditions adenylyl cyclase for beta(1)-AR stimulation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic alpha-Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-1 Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-2 Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels, L-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Cholinergic Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Epinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Norepinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, beta-1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, beta-2
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0363-6135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
279
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H1329-37
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10993800-Adrenergic Agonists,
pubmed-meshheading:10993800-Adrenergic alpha-Antagonists,
pubmed-meshheading:10993800-Adrenergic beta-1 Receptor Agonists,
pubmed-meshheading:10993800-Adrenergic beta-2 Receptor Agonists,
pubmed-meshheading:10993800-Adrenergic beta-Antagonists,
pubmed-meshheading:10993800-Animals,
pubmed-meshheading:10993800-Calcium Channels, L-Type,
pubmed-meshheading:10993800-Cells, Cultured,
pubmed-meshheading:10993800-Cholinergic Agonists,
pubmed-meshheading:10993800-Dogs,
pubmed-meshheading:10993800-Dose-Response Relationship, Drug,
pubmed-meshheading:10993800-Epinephrine,
pubmed-meshheading:10993800-Forskolin,
pubmed-meshheading:10993800-Heart Ventricles,
pubmed-meshheading:10993800-Myocardium,
pubmed-meshheading:10993800-Norepinephrine,
pubmed-meshheading:10993800-Patch-Clamp Techniques,
pubmed-meshheading:10993800-Phosphodiesterase Inhibitors,
pubmed-meshheading:10993800-Receptors, Adrenergic, beta-1,
pubmed-meshheading:10993800-Receptors, Adrenergic, beta-2
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pubmed:year |
2000
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pubmed:articleTitle |
Conditioning of beta(1)-adrenoceptor effect via beta(2)-subtype on L-type Ca(2+) current in canine ventricular myocytes.
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pubmed:affiliation |
Section of Endocrinology, Department of Internal Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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