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pubmed-article:10960690pubmed:abstractTextPrevious studies have suggested a direct relationship between the nasopharyngeal carriage of potential middle ear pathogens and the development of middle ear disease. It has been shown that otitis-prone (OP) children tend to be colonized more often than non-OP children. To study the turnover of nontypeable Haemophilus influenzae (NTHI) in the nasopharynx of OP children, arbitrarily primed PCR was applied to NTHI strains isolated nasopharyngeal swabs collected prospectively during a 2-year study period from 35 OP children under 4 years of age at fixed intervals. METHODOLOGY/MATERIALS: In 20 patients, H. influenzae could be isolated from different sites (left and/or right ear and/or nasopharynx) or at different occasions during follow-up. Forty-eight H. influenzae isolates of different sites (left and/or right ear and/or nasopharynx) of the same patient as well as from siblings were typed using arbitrarily primed PCR with primer ERIC2 and RAPD Ready-to-Go beads (Pharmacia Biotech, Uppsala, Sweden).lld:pubmed
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pubmed-article:10960690pubmed:dateRevised2004-11-17lld:pubmed
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pubmed-article:10960690pubmed:articleTitleTurnover of Haemophilus influenzae isolates in otitis-prone children.lld:pubmed
pubmed-article:10960690pubmed:affiliationDepartment of Otolaryngology, University Hospital Ghent, De Pintelaan 185, B-9000, Ghent, Belgium. ingeborg.dhooge@rug.ac.belld:pubmed
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