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rdf:type |
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|
lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2000-7-24
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pubmed:abstractText |
Ena/VASP proteins have been implicated in cell motility through regulation of the actin cytoskeleton and are found at focal adhesions and the leading edge. Using overexpression, loss-of-function, and inhibitory approaches, we find that Ena/VASP proteins negatively regulate fibroblast motility. A dose-dependent decrease in movement is observed when Ena/VASP proteins are overexpressed in fibroblasts. Neutralization or deletion of all Ena/VASP proteins results in increased cell movement. Selective depletion of Ena/VASP proteins from focal adhesions, but not the leading edge, has no effect on motility. Constitutive membrane targeting of Ena/VASP proteins inhibits motility. These results are in marked contrast to current models for Ena/VASP function derived mainly from their role in the actin-driven movement of Listeria monocytogenes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0092-8674
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
717-28
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10892743-Animals,
pubmed-meshheading:10892743-Cell Adhesion,
pubmed-meshheading:10892743-Cell Adhesion Molecules,
pubmed-meshheading:10892743-Cell Movement,
pubmed-meshheading:10892743-DNA-Binding Proteins,
pubmed-meshheading:10892743-Fibroblasts,
pubmed-meshheading:10892743-Gene Expression,
pubmed-meshheading:10892743-Gene Expression Regulation,
pubmed-meshheading:10892743-Listeria monocytogenes,
pubmed-meshheading:10892743-Microfilament Proteins,
pubmed-meshheading:10892743-Phosphoproteins
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pubmed:year |
2000
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pubmed:articleTitle |
Negative regulation of fibroblast motility by Ena/VASP proteins.
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pubmed:affiliation |
Department of Biology, Massachusetts Institute of Technology, Cambridge, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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