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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2000-8-17
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pubmed:abstractText |
Human herpes virus-8 (HHV8) encodes a cytokine named viral interleukin-6 (vIL-6) that shares 25% amino-acid identity with its human homologue. Human IL-6 is known to be a growth and differentiation factor of lymphatic cells and plays a potential role in the pathophysiology of various lymphoproliferative diseases. vIL-6 is expressed in HHV8-associated-diseases including Kaposi's sarcoma, Body-cavity-based-lymphoma and Castleman's disease, suggesting a pathogenetic involvement in the malignant growth of B-cell associated diseases and other malignant tumours. We expressed vIL-6 in Escherichia coli as a fusion protein with recombinant periplasmic maltose binding protein. After cleavage from the maltose binding protein moiety and purification, vIL-6 was shown to be correctly folded using circular dichroism spectroscopy. A rabbit antiserum was raised against the recombinant vIL-6 protein. vIL-6 turned out to be active on cells that expressed gp130 but no IL-6 receptor (IL-6-R) suggesting that, in contrast to human IL-6, vIL-6 stimulated gp130 directly. Accordingly, vIL-6 activity could be inhibited by a soluble gp130 Fc Fusion protein. vIL-6 was shown to induce neuronal differentiation of rat pheochromocytoma cells and to stimulate colony formation of human hematopoietic progenitor cells. Thus, vIL-6 exhibits biologic activity that has only been observed for the IL-6/soluble IL-6-R complex but not for IL-6 alone. These properties are important for the evaluation of the pathophysiological potential of vIL-6.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokine Receptor gp130,
http://linkedlifedata.com/resource/pubmed/chemical/GAP-43 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/IL6ST protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Il6st protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/interleukin 6-interleukin 6...
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-2956
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pubmed:author |
pubmed-author:FischerMM,
pubmed-author:GötzeK SKS,
pubmed-author:GalleP RPR,
pubmed-author:GehlAA,
pubmed-author:GrötzingerJJ,
pubmed-author:HoischenS HSH,
pubmed-author:JostockTT,
pubmed-author:MärzPP,
pubmed-author:MüllbergJJ,
pubmed-author:MechtersheimerSS,
pubmed-author:OzbekSS,
pubmed-author:PeschelCC,
pubmed-author:Rose-JohnSS,
pubmed-author:VollmerPP
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pubmed:issnType |
Print
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3604-12
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:10848977-Amino Acid Sequence,
pubmed-meshheading:10848977-Animals,
pubmed-meshheading:10848977-Antigens, CD,
pubmed-meshheading:10848977-Cell Differentiation,
pubmed-meshheading:10848977-Cell Division,
pubmed-meshheading:10848977-Cytokine Receptor gp130,
pubmed-meshheading:10848977-GAP-43 Protein,
pubmed-meshheading:10848977-Hematopoietic Stem Cells,
pubmed-meshheading:10848977-Herpesvirus 8, Human,
pubmed-meshheading:10848977-Humans,
pubmed-meshheading:10848977-Interleukin-6,
pubmed-meshheading:10848977-Membrane Glycoproteins,
pubmed-meshheading:10848977-Molecular Sequence Data,
pubmed-meshheading:10848977-Neurons,
pubmed-meshheading:10848977-PC12 Cells,
pubmed-meshheading:10848977-Rats,
pubmed-meshheading:10848977-Receptors, Interleukin,
pubmed-meshheading:10848977-Receptors, Interleukin-6,
pubmed-meshheading:10848977-Recombinant Fusion Proteins,
pubmed-meshheading:10848977-Recombinant Proteins,
pubmed-meshheading:10848977-Viral Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Human herpes virus 8 interleukin-6 homologue triggers gp130 on neuronal and hematopoietic cells.
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pubmed:affiliation |
Johannes Gutenberg University of Mainz, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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