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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0021701,
umls-concept:C0035820,
umls-concept:C0079717,
umls-concept:C0085112,
umls-concept:C0085243,
umls-concept:C0086418,
umls-concept:C0205252,
umls-concept:C0209606,
umls-concept:C0218504,
umls-concept:C0282554,
umls-concept:C0301944,
umls-concept:C0600210,
umls-concept:C0751781,
umls-concept:C1138422,
umls-concept:C1332710,
umls-concept:C1515877,
umls-concept:C1879547
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pubmed:issue |
11
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pubmed:dateCreated |
2000-6-29
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pubmed:abstractText |
Hematopoietic stem cell homing and engraftment require several adhesion interactions, which are not fully understood. Engraftment of nonobese/severe combined immunodeficiency (NOD/SCID) mice by human stem cells is dependent on the major integrins very late activation antigen-4 (VLA-4); VLA-5; and to a lesser degree, lymphocyte function associated antigen-1 (LFA-1). Treatment of human CD34(+) cells with antibodies to either VLA-4 or VLA-5 prevented engraftment, and treatment with anti-LFA-1 antibodies significantly reduced the levels of engraftment. Activation of CD34(+) cells, which bear the chemokine receptor CXCR4, with stromal derived factor 1 (SDF-1) led to firm adhesion and transendothelial migration, which was dependent on LFA-1/ICAM-1 (intracellular adhesion molecule-1) and VLA-4/VCAM-1 (vascular adhesion molecule-1). Furthermore, SDF-1-induced polarization and extravasation of CD34(+)/CXCR4(+) cells through the extracellular matrix underlining the endothelium was dependent on both VLA-4 and VLA-5. Our results demonstrate that repopulating human stem cells functionally express LFA-1, VLA-4, and VLA-5. Furthermore, this study implies a novel approach to further advance clinical transplantation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD29,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/CXCL12 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl12 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alpha4beta1,
http://linkedlifedata.com/resource/pubmed/chemical/Integrins,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Function-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fibronectin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lymphocyte Homing
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-4971
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pubmed:author |
pubmed-author:AloyMM,
pubmed-author:FranitzaSS,
pubmed-author:GrabovskyVV,
pubmed-author:KollerMM,
pubmed-author:LapidotTT,
pubmed-author:LidenKK,
pubmed-author:NaglerAA,
pubmed-author:PeledAA,
pubmed-author:PetitII,
pubmed-author:PonomaryovTT,
pubmed-author:ShaoQ FQF,
pubmed-author:ZiporiDD
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3289-96
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10828007-Animals,
pubmed-meshheading:10828007-Antibodies,
pubmed-meshheading:10828007-Antigens, CD29,
pubmed-meshheading:10828007-Antigens, CD34,
pubmed-meshheading:10828007-Cell Adhesion,
pubmed-meshheading:10828007-Cells, Cultured,
pubmed-meshheading:10828007-Chemokine CXCL12,
pubmed-meshheading:10828007-Chemokines, CXC,
pubmed-meshheading:10828007-Chemotaxis,
pubmed-meshheading:10828007-Endothelium, Vascular,
pubmed-meshheading:10828007-Fetal Blood,
pubmed-meshheading:10828007-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:10828007-Hematopoietic Stem Cells,
pubmed-meshheading:10828007-Humans,
pubmed-meshheading:10828007-Infant, Newborn,
pubmed-meshheading:10828007-Integrin alpha4beta1,
pubmed-meshheading:10828007-Integrins,
pubmed-meshheading:10828007-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:10828007-Mice,
pubmed-meshheading:10828007-Mice, Inbred NOD,
pubmed-meshheading:10828007-Mice, SCID,
pubmed-meshheading:10828007-Models, Biological,
pubmed-meshheading:10828007-Receptors, Fibronectin,
pubmed-meshheading:10828007-Receptors, Lymphocyte Homing,
pubmed-meshheading:10828007-Stromal Cells,
pubmed-meshheading:10828007-Transplantation, Heterologous
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pubmed:year |
2000
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pubmed:articleTitle |
The chemokine SDF-1 activates the integrins LFA-1, VLA-4, and VLA-5 on immature human CD34(+) cells: role in transendothelial/stromal migration and engraftment of NOD/SCID mice.
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pubmed:affiliation |
Departments of Immunology and Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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