| pubmed-article:10722685 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10722685 | lifeskim:mentions | umls-concept:C0291573 | lld:lifeskim |
| pubmed-article:10722685 | lifeskim:mentions | umls-concept:C0030012 | lld:lifeskim |
| pubmed-article:10722685 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:10722685 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:10722685 | lifeskim:mentions | umls-concept:C0036580 | lld:lifeskim |
| pubmed-article:10722685 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:10722685 | pubmed:dateCreated | 2000-4-27 | lld:pubmed |
| pubmed-article:10722685 | pubmed:abstractText | Selenium, an essential biological trace element, exerts its modulatory effects in a variety of cellular events including cell survival and death. In our study we observed that selenite protects HEK293 cells from cell death induced by ultraviolet B radiation (UVB). Exposure of HEK293 cells to UVB radiation resulted in the activation of caspase-3-like protease activity, and pretreatment of the cells with z-DEVD-fmk (N-benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethylketone), a caspase-3 inhibitor, prevented UVB-induced cell death. Interestingly, enzymatic activity of caspase-3-like protease in cell lysates of UVB-exposed cells was repressed in vitro by the presence of selenite. Selenite also inhibited the in vitro activity of purified recombinant caspase-3 in cleaving Ac-DEVD-pNA (N-acetyl-Asp-Glu-Asp-p-nitroanilide) or ICAD(L) (inhibitor of a caspase-activated deoxyribonuclease) and in the induction of DNA fragmentation. The inhibitory action of selenite on a recombinant active caspase-3 could be reversed by sulfhydryl reducing agents, such as dithiothreitol and beta-mercaptoethanol. Furthermore, pretreatment of cells with selenite suppressed the stimulation of the caspase-3-like protease activity in UVB-exposed cells, whereas dithiothreitol and beta-mercaptoethanol reversed this suppression of the enzymatic activity. Taken together, our data suggest that selenite inhibits caspase-3-like protease activity through a redox mechanism and that inhibition of caspase-3-like protease activity may be the mechanism by which selenite exerts its protective effect against UVB-induced cell death. | lld:pubmed |
| pubmed-article:10722685 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10722685 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10722685 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10722685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10722685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10722685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10722685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10722685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10722685 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10722685 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10722685 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:10722685 | pubmed:author | pubmed-author:LeeS HSH | lld:pubmed |
| pubmed-article:10722685 | pubmed:author | pubmed-author:ShioHH | lld:pubmed |
| pubmed-article:10722685 | pubmed:author | pubmed-author:KimYY | lld:pubmed |
| pubmed-article:10722685 | pubmed:author | pubmed-author:ParkI SIS | lld:pubmed |
| pubmed-article:10722685 | pubmed:author | pubmed-author:HuhS HSH | lld:pubmed |
| pubmed-article:10722685 | pubmed:author | pubmed-author:ParkH SHS | lld:pubmed |
| pubmed-article:10722685 | pubmed:author | pubmed-author:ChoiE JEJ | lld:pubmed |
| pubmed-article:10722685 | pubmed:author | pubmed-author:JungY KYK | lld:pubmed |
| pubmed-article:10722685 | pubmed:author | pubmed-author:KimI YIY | lld:pubmed |
| pubmed-article:10722685 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10722685 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:10722685 | pubmed:volume | 275 | lld:pubmed |
| pubmed-article:10722685 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10722685 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10722685 | pubmed:pagination | 8487-91 | lld:pubmed |
| pubmed-article:10722685 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:10722685 | pubmed:meshHeading | pubmed-meshheading:10722685... | lld:pubmed |
| pubmed-article:10722685 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10722685 | pubmed:articleTitle | Selenite negatively regulates caspase-3 through a redox mechanism. | lld:pubmed |
| pubmed-article:10722685 | pubmed:affiliation | National Creative Research Initiative Center for Cell Death, Korea University, Anam-dong, Sungbuk-ku, Seoul 136-701, Korea. | lld:pubmed |
| pubmed-article:10722685 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10722685 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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