Linked Life Data

10694271

Source:http://linkedlifedata.com/resource/pubmed/id/10694271

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-5-15
pubmed:abstractText
The present study was designed to determine whether progesterone might have a role in gonadotrophin-induced pituitary adenylate cyclase-activating polypeptide (Pacap) gene expression in rat ovary. Northern blot analysis revealed that treatment of pregnant mare's serum gonadotrophin (PMSG)-primed immature rats with the progestin antagonist RU486 or an inhibitor of 3beta-hydroxysteroid dehydrogenase epostane, 1 h before HCG, resulted in a dose-dependent inhibition of the HCG-induced Pacap gene expression. In-situ hybridization demonstrated that the number of pre-ovulatory follicles expressing Pacap mRNA in their granulosa cells was greatly reduced in ovaries treated with RU486. Moreover, the suppressive effect of RU486 or epostane on the LH-induced Pacap gene expression in cultured pre-ovulatory follicles was reversed by co-treatment with the synthetic progestin R5020. We further cloned the 5'-flanking region of the rat Pacap gene and identified the presence of a consensus progesterone receptor element. When luciferase fusion genes containing Pacap gene promoter were transiently transfected into granulosa cells of pre-ovulatory follicles, luciferase activity was markedly stimulated by LH. Treatment with RU486 or epostane resulted in partial suppression of LH-stimulated PACAP promoter activity. Taken together, these results indicate that progesterone, acting through progesterone receptors, plays a role in gonadotrophin induction of Pacap gene expression in granulosa cells of pre-ovulatory follicles, and thereby may be involved in the process of ovulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1360-9947
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
238-45
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10694271-Androstenols, pubmed-meshheading:10694271-Animals, pubmed-meshheading:10694271-Base Sequence, pubmed-meshheading:10694271-Cells, Cultured, pubmed-meshheading:10694271-Consensus Sequence, pubmed-meshheading:10694271-Culture Techniques, pubmed-meshheading:10694271-Female, pubmed-meshheading:10694271-Gene Expression Regulation, pubmed-meshheading:10694271-Gonadotropins, pubmed-meshheading:10694271-Granulosa Cells, pubmed-meshheading:10694271-Mice, pubmed-meshheading:10694271-Mifepristone, pubmed-meshheading:10694271-Molecular Sequence Data, pubmed-meshheading:10694271-Neuropeptides, pubmed-meshheading:10694271-Ovarian Follicle, pubmed-meshheading:10694271-Ovulation, pubmed-meshheading:10694271-Pituitary Adenylate Cyclase-Activating Polypeptide, pubmed-meshheading:10694271-Progesterone, pubmed-meshheading:10694271-Progestins, pubmed-meshheading:10694271-Promoter Regions, Genetic, pubmed-meshheading:10694271-Rats, pubmed-meshheading:10694271-Rats, Sprague-Dawley, pubmed-meshheading:10694271-Response Elements, pubmed-meshheading:10694271-Sequence Homology, Nucleic Acid, pubmed-meshheading:10694271-Transfection
pubmed:year
2000
pubmed:articleTitle
Involvement of progesterone in gonadotrophin-induced pituitary adenylate cyclase-activating polypeptide gene expression in pre-ovulatory follicles of rat ovary.
pubmed:affiliation
Hormone Research Center, Chonnam National University, Kwangju 500-757, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't