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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-3-16
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pubmed:abstractText |
The development of eosinophilia is regulated by interleukin (IL)-5. The biological specificity of eosinophilia suggests a tight and independent regulation of IL-5 expression. A number of regulatory regions in the 5'-end of the IL-5 gene have been described; many of them are involved in the regulation of other genes, and it is not clear how the specific expression of IL-5 is regulated. In this study, we report the finding of a novel 3'-regulatory element. Data base analysis of a 2-kilobase fragment of the 3'-end of the mouse IL-5 gene revealed the presence of a 40-base pair-long repetitive sequence that consists of four direct repeats of ATGAATGA distributed in a symmetrical manner. This sequence, named mouse downstream regulatory element-1 (mDRE1), was shown to be protected in DNase I footprinting assays in vitro. Electrophoretic mobility shift assays using specific antibodies identified the transcription factors Oct-1 and Oct-2 as responsible for the formation of the specific complexes with mDRE1 and nuclear extracts from both EL4 and primary T-cells. Competition electrophoretic mobility shift assays with oligonucleotides containing different numbers of ATGAATGA repeats showed that Oct-1 and Oct-2 bind to different motifs in the mDRE1 sequence. Deletion of mDRE1 from a 9.5-kilobase IL-5 gene construct significantly decreased the expression of the luciferase reporter gene, suggesting that it plays a positive role in the expression of the IL-5 gene.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Hcfc1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Host Cell Factor C1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-2,
http://linkedlifedata.com/resource/pubmed/chemical/Pou2f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pou2f2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4525-31
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:10660628-Animals,
pubmed-meshheading:10660628-Binding, Competitive,
pubmed-meshheading:10660628-Cell Line,
pubmed-meshheading:10660628-DNA Footprinting,
pubmed-meshheading:10660628-DNA-Binding Proteins,
pubmed-meshheading:10660628-Deoxyribonucleotides,
pubmed-meshheading:10660628-Eosinophilia,
pubmed-meshheading:10660628-Gene Expression Regulation,
pubmed-meshheading:10660628-Genes, Reporter,
pubmed-meshheading:10660628-Host Cell Factor C1,
pubmed-meshheading:10660628-Interleukin-5,
pubmed-meshheading:10660628-Mice,
pubmed-meshheading:10660628-Mutation,
pubmed-meshheading:10660628-Nuclear Proteins,
pubmed-meshheading:10660628-Octamer Transcription Factor-1,
pubmed-meshheading:10660628-Octamer Transcription Factor-2,
pubmed-meshheading:10660628-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:10660628-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:10660628-T-Lymphocytes,
pubmed-meshheading:10660628-Transcription Factors,
pubmed-meshheading:10660628-Transfection
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pubmed:year |
2000
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pubmed:articleTitle |
Binding of octamer factors to a novel 3'-positive regulatory element in the mouse interleukin-5 gene.
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pubmed:affiliation |
Molecular Immunology Group, School of Biomedical Sciences, Curtin University of Technology, Perth 6000, Australia.
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pubmed:publicationType |
Journal Article
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