Source:http://linkedlifedata.com/resource/pubmed/id/10640774
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2000-2-24
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| pubmed:abstractText |
Chronic rejection represents a major cause of long-term kidney graft loss. T cells that are predominant in long-term rejected kidney allografts (35 +/- 10% of area infiltrate) may thus be instrumental in this phenomenon, which is likely to be dependent on the indirect pathway of allorecognition only. We have analyzed the variations in T cell repertoire usage of the V beta chain at the complementary determining region 3 (CDR3) level in 18 human kidney grafts lost due to chronic rejection. We observed a strongly biased intragraft TCR V beta usage for the majority of V beta families and also a very high percentage (55%) of V beta families exhibiting common and oligoclonal V beta-C beta rearrangements in the grafts of patients with chronic rejection associated with superimposed histologically acute lesions. Furthermore, V beta 8 and V beta 23 families exhibited common and oligoclonal V beta-J beta rearrangements in 4 of 18 patients (22%). Several CDR3 amino acid sequences were found for the common and oligoclonal V beta 8-J beta 1.4 rearrangement. Quantitative PCR showed that biased V beta transcripts were also overexpressed in chronically rejected kidneys with superimposed acute lesions. In contrast, T lymphocytes infiltrating rejected allografts with chronic rejection only showed an unaltered Gaussian-type CDR3 length distribution. This pattern suggests that late graft failure associated with histological lesions restricted to Banff-defined chronic rejection does not involve T cell-mediated injury. Thus, our observation suggests that a limited number of determinants stimulates the recipient immune system in long-term allograft failure. The possibility of a local response against viral or parenchymatous cell-derived determinants is discussed.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
164
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1553-63
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10640774-Acute Disease,
pubmed-meshheading:10640774-Adolescent,
pubmed-meshheading:10640774-Adult,
pubmed-meshheading:10640774-Aged,
pubmed-meshheading:10640774-Cell Movement,
pubmed-meshheading:10640774-Child,
pubmed-meshheading:10640774-Chronic Disease,
pubmed-meshheading:10640774-Clone Cells,
pubmed-meshheading:10640774-Female,
pubmed-meshheading:10640774-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:10640774-Genome, Viral,
pubmed-meshheading:10640774-Graft Rejection,
pubmed-meshheading:10640774-Humans,
pubmed-meshheading:10640774-Kidney Transplantation,
pubmed-meshheading:10640774-Macrophages,
pubmed-meshheading:10640774-Male,
pubmed-meshheading:10640774-Middle Aged,
pubmed-meshheading:10640774-Monocytes,
pubmed-meshheading:10640774-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:10640774-T-Lymphocyte Subsets,
pubmed-meshheading:10640774-Transcription, Genetic
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Highly altered V beta repertoire of T cells infiltrating long-term rejected kidney allografts.
|
| pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale, Unité 437, "Immunointervention dans les Allo et Xénotransplantations" Nantes, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|