Linked Life Data

10601546

Source:http://linkedlifedata.com/resource/pubmed/id/10601546

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-1-11
pubmed:abstractText
Betacellulin (BTC) was identified in mouse pancreatic beta cell tumors as a member of the epidermal growth factor (EGF) family, and was found to bind and activate the EGF receptor. BTC is also expressed in some human malignancies and may have an important role in tumor growth progression. We examined whether BTC and EGF have a growth stimulatory effect on human pancreatic cancer cell lines both in vitro and in vivo. We also investigated the BTC expression and autonomous induction of BTC in pancreatic cancer cells. in vitro, both BTC and EGF had almost the same proliferative effect on Panc-1, MIA PaCa-2 and AsPC-1. in vivo, in a Panc-1 inoculated athymic mice model, BTC-treated tumors grew approximately five times larger than in control. Immunocytochemistry showed that BTC expression occurred in three pancreatic cancer cell lines, with MIA PaCa-2 showing the strongest intensity. Semi-quantitative RT-PCR of MIA Paca-2 showed that mRNA levels of BTC gradually increased after treatment with 1 nM BTC. Immunocytochemistry also demonstrated that the intensity of BTC-like immunoreactivity was increased when treated with 1 nM BTC but was reduced after treatment with 100 nM of AG1478, an EGF receptor tyrosine kinase inhibitor. BTC has thus a significant growth stimulatory effect on pancreatic cancer cells and might function as an autocrine and paracrine growth factor. BTC expression in pancreatic cancer cells is, at least in part, controlled by an auto-induction mechanism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1019-6439
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
37-41
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10601546-Animals, pubmed-meshheading:10601546-Cell Division, pubmed-meshheading:10601546-Epidermal Growth Factor, pubmed-meshheading:10601546-Growth Substances, pubmed-meshheading:10601546-Humans, pubmed-meshheading:10601546-Immunohistochemistry, pubmed-meshheading:10601546-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:10601546-Male, pubmed-meshheading:10601546-Mice, pubmed-meshheading:10601546-Mice, Inbred BALB C, pubmed-meshheading:10601546-Mice, Nude, pubmed-meshheading:10601546-Neoplasm Transplantation, pubmed-meshheading:10601546-Pancreatic Neoplasms, pubmed-meshheading:10601546-Receptor, Epidermal Growth Factor, pubmed-meshheading:10601546-Recombinant Proteins, pubmed-meshheading:10601546-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10601546-Tumor Cells, Cultured
pubmed:year
2000
pubmed:articleTitle
Auto-induction and growth stimulatory effect of betacellulin in human pancreatic cancer cells.
pubmed:affiliation
Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't