Source:http://linkedlifedata.com/resource/pubmed/id/10586881
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6760
|
| pubmed:dateCreated |
1999-12-10
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| pubmed:databankReference | |
| pubmed:abstractText |
Economical methods by which gene function may be analysed on a genomic scale are relatively scarce. To fill this need, we have developed a transposon-tagging strategy for the genome-wide analysis of disruption phenotypes, gene expression and protein localization, and have applied this method to the large-scale analysis of gene function in the budding yeast Saccharomyces cerevisiae. Here we present the largest collection of defined yeast mutants ever generated within a single genetic background--a collection of over 11,000 strains, each carrying a transposon inserted within a region of the genome expressed during vegetative growth and/or sporulation. These insertions affect nearly 2,000 annotated genes, representing about one-third of the 6,200 predicted genes in the yeast genome. We have used this collection to determine disruption phenotypes for nearly 8,000 strains using 20 different growth conditions; the resulting data sets were clustered to identify groups of functionally related genes. We have also identified over 300 previously non-annotated open reading frames and analysed by indirect immunofluorescence over 1,300 transposon-tagged proteins. In total, our study encompasses over 260,000 data points, constituting the largest functional analysis of the yeast genome ever undertaken.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0028-0836
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| pubmed:author |
pubmed-author:AgarwalSS,
pubmed-author:CheungK HKH,
pubmed-author:CoelhoP SPS,
pubmed-author:GersteinMM,
pubmed-author:HagerKK,
pubmed-author:HeidtmanMM,
pubmed-author:IwasakiHH,
pubmed-author:JansenRR,
pubmed-author:KumarAA,
pubmed-author:MillerPP,
pubmed-author:NelsonF KFK,
pubmed-author:RoederG SGS,
pubmed-author:RoemerTT,
pubmed-author:Ross-MacdonaldPP,
pubmed-author:SheehanAA,
pubmed-author:SnyderMM,
pubmed-author:SymoniatisDD,
pubmed-author:UmanskyLL
|
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
402
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
413-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:10586881-Algorithms,
pubmed-meshheading:10586881-DNA Transposable Elements,
pubmed-meshheading:10586881-Escherichia coli,
pubmed-meshheading:10586881-Fungal Proteins,
pubmed-meshheading:10586881-Gene Expression Profiling,
pubmed-meshheading:10586881-Genetic Techniques,
pubmed-meshheading:10586881-Genome, Fungal,
pubmed-meshheading:10586881-Molecular Sequence Data,
pubmed-meshheading:10586881-Mutagenesis,
pubmed-meshheading:10586881-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:10586881-Open Reading Frames,
pubmed-meshheading:10586881-Phenotype,
pubmed-meshheading:10586881-Polymerase Chain Reaction,
pubmed-meshheading:10586881-Saccharomyces cerevisiae,
pubmed-meshheading:10586881-Transformation, Genetic
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Large-scale analysis of the yeast genome by transposon tagging and gene disruption.
|
| pubmed:affiliation |
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|