Linked Life Data

10556311

Source:http://linkedlifedata.com/resource/pubmed/id/10556311

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2000-1-14
pubmed:abstractText
Gut-enriched Krüppel-like factor (GKLF, KLF4) is an epithelial-specific transcription factor whose expression is associated with growth arrest. In order to understand the mechanisms regulating expression of the gene encoding GKLF, we isolated a genomic clone containing murine GKLF. The gene spans 5.3 kb and contains four exons. A major start site of transcription was mapped to an adenine residue 601 nt 5' of the translation initiation codon. An additional 1 kb of the 5'-flanking region was sequenced and found to contain multiple cis -elements homologous to the binding sites of several established transcription factors including Sp1, AP-1, Cdx, GATA, and USF. In particular, three closely spaced GC-boxes 5' of the TATA box resemble the established binding site for GKLF. DNase I protection and electrophoretic mobility shift assays verified that recombinant GKLF bound to each of the three GC-boxes. In co-transfection experiments, GKLF transactivated a reporter gene linked to the GKLF 1 kb 5'-flanking region, as did Sp1, Sp3 and Cdx-2. Mutations of one or both of the first and second GC-boxes in the promoter resulted in diminished transactivation by GKLF. These results demonstrate that the 5'-flanking sequence of the mouse GKLF gene functions as a promoter and is subject to autoregulation by its own gene product.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4562-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10556311-Animals, pubmed-meshheading:10556311-Base Sequence, pubmed-meshheading:10556311-CHO Cells, pubmed-meshheading:10556311-COS Cells, pubmed-meshheading:10556311-Cricetinae, pubmed-meshheading:10556311-DNA Footprinting, pubmed-meshheading:10556311-DNA-Binding Proteins, pubmed-meshheading:10556311-Gene Expression Regulation, pubmed-meshheading:10556311-Kruppel-Like Transcription Factors, pubmed-meshheading:10556311-Mice, pubmed-meshheading:10556311-Molecular Sequence Data, pubmed-meshheading:10556311-Mutagenesis, Site-Directed, pubmed-meshheading:10556311-Promoter Regions, Genetic, pubmed-meshheading:10556311-Recombinant Proteins, pubmed-meshheading:10556311-Transcription Factors, pubmed-meshheading:10556311-Transcriptional Activation, pubmed-meshheading:10556311-Zinc Fingers
pubmed:year
1999
pubmed:articleTitle
Characterization of the structure and regulation of the murine gene encoding gut-enriched Krüppel-like factor (Krüppel-like factor 4).
pubmed:affiliation
Department of Medicine, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.