Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2000-1-18
pubmed:abstractText
Glucosinolates, a group of naturally occurring thioglucosides, are significant factors impairing the nutritional quality of rapeseed and postextraction rapeseed meal, restricting its use as high-quality protein animal feed. Currently, the European Community standards and Canola definition are being brought in line recommending cultivation and marketing of rapeseed with a glucosinolate content below 18 micromol of total glucosinolates per gram of seeds. Furthermore, some glucosinolates are of increasing interest in Brassica vegetables due to their proven cancer-preventing activities. A novel approach to the analysis of total glucosinolates is reported in this paper based on their alkaline degradation and subsequent reaction of released 1-thioglucose with ferricyanide. The reaction was followed spectrophotometrically using sinigrin and glucotropeaolin as model glucosinolates. The applicability of the method was demonstrated using rapeseed extracts after reducing the interfering effect of phenolics by their adsorption onto polyvinylpolypyrrolidone. Good agreement with official ISO methods was shown.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-8561
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4669-74
pubmed:dateRevised
2010-1-22
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Determination of glucosinolates using their alkaline degradation and reaction with ferricyanide.
pubmed:affiliation
Sensor & Cryobiology Research Group, The Research Centre, University of Luton, The Spires, 2 Adelaide Street, Luton LU1 5DU, United Kingdom.
pubmed:publicationType
Journal Article