Source:http://linkedlifedata.com/resource/pubmed/id/10527617
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1999-12-7
|
pubmed:abstractText |
Angiogenesis involves proliferation of capillary endothelial cells and formation of lumen-containing tube-like structures. A recently established murine brain capillary endothelial cell line, IBE, can either proliferate or form tube-like structures (i.e., differentiate) in response to fibroblast growth factor-2 (FGF-2), dependent on the culture conditions. The 4N1K peptide (KRFYVVMWKK), which is derived from the C-terminal cell-binding domain of thrombospondin-1 (TSP-1), inhibited tube formation, but not proliferation of IBE cells. Polyclonal antibodies against 4N1K blocked TSP-1-induced inhibition of tube formation by IBE cells. 4N1K inhibited tyrosine phosphorylation of focal adhesion kinase and FGF-2-stimulated tyrosine phosphorylation of phospholipase C-gamma in tube-forming, but not proliferating, IBE cells. The peptide also inhibited FGF-2-induced neovascularization in mouse cornea. Our results indicate that TSP-1 may exert its inhibitory effects on angiogenesis via the C-terminal cell-binding domain containing the 4N1K sequence by inhibiting tube formation by endothelial cells.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0014-4827
|
pubmed:author | |
pubmed:copyrightInfo |
Copyright 1999 Academic Press.
|
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
252
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
262-72
|
pubmed:dateRevised |
2004-11-17
|
pubmed:meshHeading |
pubmed-meshheading:10527617-Animals,
pubmed-meshheading:10527617-Cell Differentiation,
pubmed-meshheading:10527617-Endothelium, Vascular,
pubmed-meshheading:10527617-Fibroblast Growth Factor 2,
pubmed-meshheading:10527617-Humans,
pubmed-meshheading:10527617-Mice,
pubmed-meshheading:10527617-Morphogenesis,
pubmed-meshheading:10527617-Neovascularization, Physiologic,
pubmed-meshheading:10527617-Oligopeptides,
pubmed-meshheading:10527617-Thrombospondin 1
|
pubmed:year |
1999
|
pubmed:articleTitle |
Role of thrombospondin-1-derived peptide, 4N1K, in FGF-2-induced angiogenesis.
|
pubmed:affiliation |
Department of Urology, Nagasaki University School of Medicine, Nagasaki, Japan.
|
pubmed:publicationType |
Journal Article
|