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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1999-10-12
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pubmed:abstractText |
BrkA is a 103-kDa outer membrane protein of Bordetella pertussis that mediates resistance to antibody-dependent killing by complement. It is proteolytically processed into a 73-kDa N-terminal domain and a 30-kDa C-terminal domain as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. BrkA is also a member of the autotransporter family of proteins. Translocation of the N-terminal domain of the protein across the outer membrane is hypothesized to occur through a pore formed by the C-terminal domain. To test this hypothesis, we performed black lipid bilayer experiments with purified recombinant protein. The BrkA C-terminal protein showed an average single-channel conductance of 3.0 nS in 1 M KCl. This result strongly suggests that the C-terminal autotransporter domain of BrkA is indeed capable of forming a pore.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10482528-10022352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10482528-10026305,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10482528-9886293
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
181
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5838-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10482528-Bacterial Outer Membrane Proteins,
pubmed-meshheading:10482528-Bordetella pertussis,
pubmed-meshheading:10482528-Cloning, Molecular,
pubmed-meshheading:10482528-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10482528-Escherichia coli,
pubmed-meshheading:10482528-Lipid Bilayers,
pubmed-meshheading:10482528-Liposomes,
pubmed-meshheading:10482528-Peptide Fragments,
pubmed-meshheading:10482528-Recombinant Proteins,
pubmed-meshheading:10482528-Restriction Mapping,
pubmed-meshheading:10482528-Species Specificity
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pubmed:year |
1999
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pubmed:articleTitle |
The C-terminal domain of the Bordetella pertussis autotransporter BrkA forms a pore in lipid bilayer membranes.
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pubmed:affiliation |
Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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