Linked Life Data

10428917

Source:http://linkedlifedata.com/resource/pubmed/id/10428917

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1999-9-9
pubmed:abstractText
Acyclovir (ACV) has shown efficacy in the prophylactic suppression of human cytomegalovirus (HCMV) reactivation in immunocompromised renal transplant patients without the toxicity associated with ganciclovir (GCV). The HCMV UL97 gene product, a protein kinase, is responsible for the phosphorylation of GCV in HCMV-infected cells. This report provides evidence for the phosphorylation of ACV by UL97. Anabolism studies with the HCMV wild-type strain AD169 and with recombinant mutants derived from marker transfer experiments performed by using mutant UL97 DNA from both clinical isolates and a laboratory-derived strain resistant to GCV showed that mutations in the UL97 gene cripple the ability of recombinant virus-infected cells to anabolize both GCV and ACV. These mutant UL97 recombinant viruses were less susceptible to both GCV and ACV than was the wild-type strain. A recombinant herpes simplex virus type 1 strain, in which the thymidine kinase gene is deleted and the UL13 gene is replaced with the HCMV UL97 gene, was able to induce the phosphorylation of ACV in infected cells. Finally, purified UL97 phosphorylated both GCV and ACV to their monophosphates. Our results indicate that UL97 promotes the selective activity of ACV against HCMV.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-1319559, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-1319560, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-1654361, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-1654362, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-1666492, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-1849157, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-202961, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-214430, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-2173786, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-232189, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-2541335, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-2827025, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-2849621, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-2986118, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-2991214, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-3022304, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-3032503, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6090044, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6092331, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6248718, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6264640, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6281874, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6282196, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6285735, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6306664, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6307132, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6313660, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-6316844, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-7625819, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-7815545, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-7859141, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-7876604, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-7966639, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-7979285, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-8207815, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-8267423, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-8709262, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-8874075, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-8918921, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-8985364, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-9330759, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-9419169, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-9419170, http://linkedlifedata.com/resource/pubmed/commentcorrection/10428917-9527804
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0066-4804
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1941-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Acyclovir is phosphorylated by the human cytomegalovirus UL97 protein.
pubmed:affiliation
Department of Virology, Glaxo Wellcome, Inc., Research Triangle Park, North Carolina 27709, USA. clt39226@glaxowellcome.com
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.